肝素酶产生菌的筛选及发酵条件

从土壤中筛选到一株活性较高的肝素酶产生菌株Corynebacterium sp.。培养及产酶最佳条件表明，最适培养基组成(g/L)：胰蛋白胨20，氯化钠1，磷酸氢二钾25，硫酸镁05，麦芽糖20，pH65。最适生长温度27℃，最佳产酶温度31℃。在500mL三角瓶中装40mL培养基，在30℃，200r/min摇床上培养24 h，每升发酵液可产酶1700u。

STRAIN SCREENING AND FERMENTATION CONDITIONS OF A NOVEL HEPARINASE PRODUCING STRAIN

The novel heparinase-producing bacterial strain Corynebacterium sp. was screened and isolated from soil. The optimum medium composition is (g/L): Trypticase 20, NaCl 1, K2HPO4 2.5, MgSO4 0.5, Heparin 2, maltose 20, pH 6.5. The optimum growth temperature was 27 degrees C while, maximum enzyme production was achieved at temperature 31 degrees C. When cultured at a rotating shaker at 30 degrees C for 24 hours, 200 r/min, 40 mL medium in 500 mL flask, the Production of heparinase reached 1700 u/L.