| 结核分枝杆菌中插入序列的研究 |
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| 引用本文： | 蔡宏,李君莲,武坚,张曼夫,陈永福.结核分枝杆菌中插入序列的研究[J].微生物学报,1999,39(2):120-126. |
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| 作者姓名： | 蔡宏 李君莲 武坚 张曼夫 陈永福 |
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| 作者单位： | 新疆畜牧科学院兽医研究所 乌鲁木齐 830000；新疆结核病研究所 乌鲁木齐 830001；新疆畜牧科学院农业部畜牧兽医生物技术重点开
放实验室 乌鲁木齐830000；中国农业大学生物学院 北京 100094；中国农业大学农业生物技术国家重点实验室 北
京 100094 |
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| 摘 要： | 用人型复合分枝杆菌的特异插入序列IS6110和IS1081制
备探针，对5种限制性内切酶消化的结核分枝杆菌DNA进行杂交。结果表明，经PvuⅡ酶消化
的结核分枝杆菌DNA，用IS6110制备的探针进行杂交呈现高度多态性，说明IS6110对于人型
复合分枝杆菌分型研究和结核病流行病学研究具有很大价值。用IS6110制备的317bp探针对4
6株人结核分枝杆菌分离株多态性分析研究证实，这些菌株呈现高度DNA多态性，而且所含拷
贝数也极为不同，一般含7～18个拷贝。
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| 关 键 词： | 结核分枝杆菌，插入序列，限制性酶切片段长度多态性(RFLP) |
SPECIES IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS WITH INSERTION
ELEMENTS |
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| Cai Hong.SPECIES IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS WITH INSERTION
ELEMENTS[J].Acta Microbiologica Sinica,1999,39(2):120-126. |
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| Authors: | Cai Hong |
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| Institution: | Xinjiang Academy of Animal Science, Institute of Veterinary, Urümqi 830000. |
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| Abstract: | The Insertion Sequence(IS) element IS6110 and IS1081 of M. tuberculosis complex were used as template to prepare probes for typing strains by means of restriction fragment length polymorphisms (RFLP). Chromosomal DNA of M. tuberculosis H37Ra, H37Rv, M. bovis digested with EcoR I, Pst I, Sal I, BamH I, Pvu II was subjected to Southern Blot analysis With labeled 317bp from IS6110 and 248 bp from IS1081. The results indicated that for M. tuberculosis complex, IS1081 cannot be used to type strains, IS6110 can be used to distinguish strains into broad groups and is an excellent epidemiological tool for studying outbreaks. An investigation of forty-six M. tuberculosis isolates by IS6110 RFLP revealed that M. tuberculosis strains not only are of highly polymorphic, but also are presentin multiple copies ranging from 7 to 18 copies. |
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| Keywords: | M tuderculosis Insertion sequence(IS) RFLP |
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