| 微波诱变结合化学诱变选育酸性蛋白酶高产菌 |
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| 引用本文： | 李永泉,翁醒华,贺筱蓉.微波诱变结合化学诱变选育酸性蛋白酶高产菌[J].微生物学报,1999,39(2):181-184. |
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| 作者姓名： | 李永泉 翁醒华 贺筱蓉 |
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| 作者单位： | 浙江大学生命科学学院 杭州 310012 |
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| 摘 要： | 酸性蛋白酶是酶制剂工业比较重要的酶种，广泛应用于饲料、食品、皮革、医药和酿造工业中［１～４］，具有较大的市场潜力。本研究以宇佐美曲霉（Ａｓｐｅｒｇｉｌｕｓｕｓａｍｉ）白色突变株Ｂ１为出发菌株，通过微波诱变和亚硝基胍、硫酸锂复合诱变剂点试平板法诱变，选...
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| 关 键 词： | 微波诱变，化学诱变，宇佐美曲霉，酸性蛋白酶 |
STUDIES ON THE SCREENING HIGH YIELD ACID PROTEASE PRODUCING STRAIN L336 BY COMBINING MICROWAVE IRRADIATION WITH CHEMICAL INDUCING |
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| Li Yongquan\ Weng Xinghua\ He Xiaorong.STUDIES ON THE SCREENING HIGH YIELD ACID PROTEASE PRODUCING STRAIN L336 BY COMBINING MICROWAVE IRRADIATION WITH CHEMICAL INDUCING[J].Acta Microbiologica Sinica,1999,39(2):181-184. |
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| Authors: | Li Yongquan\ Weng Xinghua\ He Xiaorong |
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| Institution: | College of Life Sciences, Zhejiang University, Hangzhou 310012. |
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| Abstract: | A high yield acid protease producing strain Aspergillus usamii L336 was screened from its parent strain B1 by combining microwave irradiation with inducing of nitrosoguanidine and Li2SO4. By incubation of L336 in shaking flask at 31 degrees C for 88 h, its enzyme activity was 5500 u/mL, and its properties for high yield producing acid protease remained stable after many times of subculture and storage for two months. |
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| Keywords: | Microwave irradiation Chemical inducing Aspergrillus usamii L336 Acid protease |
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