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| 抗性库蚊酯酶基因在大肠杆菌中的克隆和表达 | |
| 引用本文： | 闫艳春,乔传令,尚宏宇,钱传范.抗性库蚊酯酶基因在大肠杆菌中的克隆和表达[J].微生物学报,2000,40(2):126-131. |
| 作者姓名： | 闫艳春 乔传令 尚宏宇 钱传范 |
| 作者单位： | 1. 山东农业大学生命科学学院,泰安,271018;中国科学院动物研究所,北京,100080 2. 中国科学院动物研究所,北京,100080 3. Madison WI53703 USA 4. 中国农业大学应用化学系,北京,100094 |
| 基金项目： | 国家自然科学基金资助项目(39680001)；山东省自然科学基金资助课题(Y98D16064) |
| 摘 要： | 用抗性库蚊酯酶基因,引入原核表达载体pRL439,转化大肠杆菌HB101细胞,获得表达。通过酶切、Southern杂交鉴定重组质粒。研究了重组菌酯酶的活性,重组质粒pRLB1表达的酯酶具有高酶活并能高效降解酯酶的特异性底物α乙酸萘酯(αNA)和β乙酸萘酯(βNA);经对重组菌进行细胞固定化后降解农药三氯杀虫酯(7504),反应时间短,降解效率高 |
| 关 键 词： | 酯酶基因 基因表达 大肠杆菌 降解农药 |
| 文章编号： | 0001-6209(2000)02-0126-31 |
CLONING AND EXPRESSION OF A RESISTANT CULEX GENE IN ESCHERICHIA COLI |
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| Yan Yuanchun,Qiao Chuanling,Shang Hongyu,Qian Chuanfan.CLONING AND EXPRESSION OF A RESISTANT CULEX GENE IN ESCHERICHIA COLI[J].Acta Microbiologica Sinica,2000,40(2):126-131. | |
| Authors: | Yan Yuanchun Qiao Chuanling Shang Hongyu Qian Chuanfan |
| Institution: | Yan Yuanchun ;(Life Sciences of College, Shandong Agricultural University, Taian 271018);Yan Yuanchun ,Qiao Chuanling ;(Institute of Zoology, Chinese Academy of Sciences, Beijing 100080);Shang Hongyu ;(Madison, WI 53703, USA);Qian Chuanfan ;(China Agricultural University, Beijing 100094) |
| Abstract: | Recombinant plasmid pRLB1 was constructed from detoxifying gene B1 of pesticide resistant Culex pipiens quinquefasciatus and from plasmid pRL439 contained the strong promoter PpsbA. The positive clone was identified by digestion and Southern analysis. Expression of recombinant plasmid containing esterase gene was detected. An engineered bacterium possessing high enzyme activity was obtained and immobilized. It can effectively degrade the specific substrate alpha-naphthyl acetate (alpha-NA) and beta-naphthyl of esterase enzyme. Assays showed that pesticide acetofenate (7504 an organic choride pesticide) was degraded by the immobilized cells within one hour. |
| Keywords: | Esterase gene Gene expression Escherichia coli Degradation pesticide |
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