| 亚稀褶黑菇和稀褶黑菇的ITS序列分析 |
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| 引用本文： | 尹军华,张平,龚庆芳,陈作红.亚稀褶黑菇和稀褶黑菇的ITS序列分析[J].微生物学报,2008,27(2):237-242. |
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| 作者姓名： | 尹军华 张平 龚庆芳 陈作红 |
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| 作者单位： | College of Life Sciences, Hunan Normal University, Changsha 410081, China;College of Life Sciences, Hunan Normal University, Changsha 410081, China;College of Life Sciences, Hunan Normal University, Changsha 410081, China;College of Life Sciences, Hunan Normal University, Changsha 410081, China |
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| 基金项目： | 国家自然科学基金（No. 30471208） |
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| 摘 要： | 对亚稀褶黑菇和稀褶黑菇的ITS全序列进行了测定和比较，首次报道了亚稀褶黑菇的ITS1和5.8S区域。在ITS区域中，不同采集地的亚稀褶黑菇与稀褶黑菇的5.8S rDNA具有100%的同源性，而两侧ITS之间表现出种内和种间的多态性，种内的差异均不超过5%，种间的差异达10%左右。ITS序列分析方法可以作为两者的鉴定方法。
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| 关 键 词： | rDNA，多态性，红菇属，鉴定方法 |
Sequence analysis of the internal transcribed spacer of gene coding for rDNA in Russula subnigricans and R. nigricans |
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| YIN Jun-Hu,ZHANG Ping,GONG Qing-Fang and CHEN Zuo-Hong.Sequence analysis of the internal transcribed spacer of gene coding for rDNA in Russula subnigricans and R. nigricans[J].Acta Microbiologica Sinica,2008,27(2):237-242. |
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| Authors: | YIN Jun-Hu ZHANG Ping GONG Qing-Fang and CHEN Zuo-Hong |
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| Abstract: | The internal transcribed spacer of gene coding for rDNA of Russula subnigricans and Russula nigricans were sequenced and analyzed, and 5.8S rDNA sequences and ITS1 sequences of R. subnigricans were reported for the first time. The results show that there is no difference between 5.8S rDNA sequences of R. subnigricans and R. nigricans, but the length discrepancy and sequence polymorphism of ITS1 and ITS2 are existed between the two species. Their intraspecies distinctions did not exceed 5%, but their interspecies distinctions were about 10%. The ITS sequence can be used as identification method for R. subnigricans and R. nigricans. |
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| Keywords: | rDNA polymorphism Russula identification method |
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