| 过氧化氢胁迫促进产朊假丝酵母合成谷胱甘肽 |
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| 引用本文： | 廖鲜艳,张文燕,朱至,陈坚,堵国成.过氧化氢胁迫促进产朊假丝酵母合成谷胱甘肽[J].微生物学报,2008,24(6):1046-1050. |
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| 作者姓名： | 廖鲜艳 张文燕 朱至 陈坚 堵国成 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室, 无锡 214122; 江南大学生物工程学院生物系统与生物加工工程研究室, 无锡 214122;江南大学生物工程学院生物系统与生物加工工程研究室, 无锡 214122;江南大学工业生物技术教育部重点实验室, 无锡 214122; 江南大学生物工程学院生物系统与生物加工工程研究室, 无锡 214122;江南大学工业生物技术教育部重点实验室, 无锡 214122; 江南大学生物工程学院生物系统与生物加工工程研究室, 无锡 214122;江南大学工业生物技术教育部重点实验室, 无锡 214122; 江南大学生物工程学院生物系统与生物加工工程研究室, 无锡 214122 |
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| 基金项目： | 国家863项目(No. 2006AA10Z31)资助。 |
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| 摘 要： | 谷胱甘肽(GSH)在生物细胞抵御外界环境条件的刺激和胁迫时起到非常重要的作用。考察了不同时间不同浓度过氧化氢胁迫和过氧化氢连续胁迫对产朊假丝酵母合成GSH的影响, 发现低浓度过氧化氢的连续胁迫对GSH的合成有明显促进作用。进一步在发酵罐上应用了低浓度过氧化氢(36 mmol/L)持续胁迫策略, 最终GSH产量为922 mg/L, 胞内GSH含量为1.64%, 比对照分别提高了7%和35%。
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| 关 键 词： | 过氧化氢 胁迫 产朊假丝酵母 谷胱甘肽 |
Effect of H2O2 Stress on Glutathione Production by Candida utilis |
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| Xianyan Liao,Wenyan Zhang,Zhi Zhu,Jian Chen and Guocheng Du.Effect of H2O2 Stress on Glutathione Production by Candida utilis[J].Acta Microbiologica Sinica,2008,24(6):1046-1050. |
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| Authors: | Xianyan Liao Wenyan Zhang Zhi Zhu Jian Chen and Guocheng Du |
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| Institution: | Key Laboratory Industrial Biotechonology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Laboratory Biosystem & Bioprocess Engineering, School of Biotechnology, Jiangnan University, Wuxi 214122, China;Laboratory Biosystem & Bioprocess Engineering, School of Biotechnology, Jiangnan University, Wuxi 214122, China;Key Laboratory Industrial Biotechonology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Laboratory Biosystem & Bioprocess Engineering, School of Biotechnology, Jiangnan University, Wuxi 214122, China;Key Laboratory Industrial Biotechonology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Laboratory Biosystem & Bioprocess Engineering, School of Biotechnology, Jiangnan University, Wuxi 214122, China;Key Laboratory Industrial Biotechonology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Laboratory Biosystem & Bioprocess Engineering, School of Biotechnology, Jiangnan University, Wuxi 214122, China |
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| Abstract: | Glutathione (GSH) plays an important role in the responses of microorganisms to the environmental stimulation and stress. The effect of H2O2 stress under different fermentation time and H2O2 concentration as well as continuous stress on GSH fermentation of Candida utilis were investigated in this paper. It was found that low concentration of H2O2 accelerated GSH production. When treated by low concentration of H2O2 (36 mmol/L), the final concentration of GSH reached 922 mg/L and the intracellular GSH content reached 1.64%, which increased by 7% and 35% than the controls, respectively. |
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| Keywords: | H2O2 stress Candida utilis glutathione |
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