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高温链霉菌24#的初步研究
引用本文:李德舜,苏忠锐,孙琦,郭新平,马井玉.高温链霉菌24#的初步研究[J].微生物学报,2004,44(5):580-583.
作者姓名:李德舜  苏忠锐  孙琦  郭新平  马井玉
作者单位:1. 山东大学微生物技术国家重点实验室,济南,250100
2. 山东省济宁市农业科学院,济宁,272100
摘    要:从土壤中筛选得到一株产广谱、高活性抗真菌物质的链霉菌 2 4 # ,经测定对 2 5种植物病原真菌有显著拮抗作用 ,其次生代谢产物对病菌菌丝有断裂、扭曲、缢缩等致畸效应。 16SrDNA序列分析显示本菌株与模式菌株链霉菌DSM4 4 2 93T的 16SrDNA同源性为 98 93% ,但菌株形态特征、培养特征、细胞壁化学组分、生理生化特性等均不同于模式菌株 ,且DNA杂交率只有 2 2 5 4 % ,建议为链霉菌属的一个新种 ,命名为山东链霉菌 (Streptomycesshandon gensissp .nov .)。

关 键 词:山东链霉菌  抗真菌活性  DNA-DNA杂交  鉴定  16SrDNA序列
文章编号:0001-6209(2004)05-0580-04
修稿时间:2004年1月6日

A Preliminary Study on the Thermostreptomyces Strain 24 #
LI De-Shun.A Preliminary Study on the Thermostreptomyces Strain 24 #[J].Acta Microbiologica Sinica,2004,44(5):580-583.
Authors:LI De-Shun
Institution:LI De-Shun~
Abstract:The 24# strain of Streptomyces sp. producing broad-scope,high activities and antifungal substances were isolated from the soil. In screening process of agriculture antagonistic strains, streptomyces strain 24# was isolated from soil in Shandong. The antifungal substances have notable antagonistic effects of phytopathogens and can cause the mycelia of the phytopathogens breaking, twisting and constricting. The 16S rDNA sequence homology between the strain 24# and model strain S. DSM44293~(T) is 98.93%. However, the morphological and culture characteristics, the chemical components of the cell walls, the physiological and biochemical characteristics of the strain are different to those of the model strain, and the ratio of the DNA-DNA hybridization is only 22.54%. The name Streptomyces shandongensis sp. nov. is proposed for strain 24#.
Keywords:Streptomyces shandongensis sp  nov    Antifungal activity  DNA-DNA hybridization  Identification  (16S rDNA) sequence  
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