| 山羊M II期卵母细胞胞质支持异种间克隆胚的着床前发育 |
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| 引用本文： | 徐旭俊,刘国辉,陈建泉,陈娟,沙红英,吴友兵,张爱民,成国祥.山羊M II期卵母细胞胞质支持异种间克隆胚的着床前发育[J].微生物学报,2008,24(3):430-435. |
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| 作者姓名： | 徐旭俊 刘国辉 陈建泉 陈娟 沙红英 吴友兵 张爱民 成国祥 |
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| 作者单位： | 上海转基因研究中心, 上海 201203, 同济大学生命科学与技术学院, 上海 200092;上海转基因研究中心, 上海 201203;上海转基因研究中心, 上海 201203;上海转基因研究中心, 上海 201203, 同济大学生命科学与技术学院, 上海 200092;上海转基因研究中心, 上海 201203;上海转基因研究中心, 上海 201203, 同济大学生命科学与技术学院, 上海 200092;同济大学生命科学与技术学院, 上海 200092;上海转基因研究中心, 上海 201203, 同济大学生命科学与技术学院, 上海 200092 |
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| 基金项目： | 国家自然科学基金 (No.30370703)资助。 |
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| 摘 要： | 研究去核山羊(Capra hircus)体内成熟的M II期卵母细胞与异种成年的哺乳动物(包括山羊、波尔山羊、牛、塔尔羊、熊猫)及人的成纤维细胞融合形成的体细胞核移植胚胎着床前的发育能力。结果显示这些异种体细胞核移植重构胚可以完成着床前发育, 并形成囊胚。种内体细胞核移植胚的融合率和囊胚发育率分别为78.67%(557/708)和56.29%(264/469); 亚种间或种间体细胞核移植胚的融合率和囊胚发育率分别为: 波尔山羊78.18%(541/692)、33.90%(40/118), 牛70.53%(146/207)、22.52%(25/111), 塔尔羊53.51%(61/114)、5.26%(3/570), 熊猫79.82%(1159/1452)、8.35%(75/898), 人68.76%(317/461)、5.41%(16/296)。由此结果得出以下结论: (1)山羊M II期卵母细胞胞质与供核细胞之间的亲缘性不影响两者的融合率; (2)山羊M II期卵母细胞的胞质能支持异种间体细胞核移植胚的着床前发育; (3)亲缘关系近的种间核移植胚的囊胚发育率高于亲缘关系远的种间核移植胚的。
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| 关 键 词： | 山羊 中II期卵母细胞 胞质 异种间体细胞核移植胚 发育能力 |
| 收稿时间： | 7/3/2007 12:00:00 AM |
Goat MII Ooplasts Support Preimplantation Development of Embryos Cloned from Other Species |
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| Xujun Xu,Guohui Liu,Jianquan Chen,Juan Chen,Hongying Sh,Youbing Wu,Aimin Zhang and Guoxiang Cheng.Goat MII Ooplasts Support Preimplantation Development of Embryos Cloned from Other Species[J].Acta Microbiologica Sinica,2008,24(3):430-435. |
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| Authors: | Xujun Xu Guohui Liu Jianquan Chen Juan Chen Hongying Sh Youbing Wu Aimin Zhang and Guoxiang Cheng |
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| Institution: | Shanghai Transgenic Research Center, Shanghai 201203, China, School of Life Science and Technology, Tongji University, Shanghai 200092, China;Shanghai Transgenic Research Center, Shanghai 201203, China;Shanghai Transgenic Research Center, Shanghai 201203, China;Shanghai Transgenic Research Center, Shanghai 201203, China, School of Life Science and Technology, Tongji University, Shanghai 200092, China;Shanghai Transgenic Research Center, Shanghai 201203, China;Shanghai Transgenic Research Center, Shanghai 201203, China;School of Life Science and Technology, Tongji University, Shanghai 200092, China;Shanghai Transgenic Research Center, Shanghai 201203, China, School of Life Science and Technology, Tongji University, Shanghai 200092, China |
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| Abstract: | The preimplantation development competences of somatic cell nuclear transfer (SCNT) embryos reconstructed with enuleated goat (Capra hircus) Metaphase II (MII) oocytes matured in vivo and whole cells derived from adult fibroblasts of several mammalian species (goat, boer goat, bovine, tahr, panda) and human patient were evaluated. Results obtained from our experiments revealed that these reconstructed SCNT embryos could complete preimplantation development to form blastocysts. The fusion rate and blastocyst rate of intra-species SCNT embryos (Capra hircus as control) was 78.67 (557/708); 56.29% (264/469), that of sub-species or inter-species SCNT embryos were: boer goat 78.18% (541/692); 33.90% (40/118), bovine 70.53% (146/207); 22.52% (25/111), tahr 53.51% (61/114); 5.26% (3/570), panda 79.82% (1159/1452); 8.35% (75/898) and human 68.76% (317/461); 5.41% (16/296), respectively. It is concluded that (1) there are no relationships between fusion rate and relativeness of the recipient cytoplasm to nucleus donor cells, (2) cytoplast of the goat MII oocyte can support the preimplantation development of SCNT embryos reconstructed with nucleus from other species, (3) the blastocyst rate of close relative inter-species SCNT embryos is higher than that of distant relative inter-species SCNT embryos. |
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| Keywords: | goat MII oocyte cytoplast interspecies SCNT embryos developmental competence |
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