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| 六种检测猪瘟病毒方法的比较 | |
| 引用本文： | 王向鹏,张兴娟,孙元,李娜,贺番,常天明,李宏宇,杨增岐,仇华吉.六种检测猪瘟病毒方法的比较[J].微生物学报,2010,50(8):1087-1093. |
| 作者姓名： | 王向鹏 张兴娟 孙元 李娜 贺番 常天明 李宏宇 杨增岐 仇华吉 |
| 作者单位： | 1. 西北农林科技大学动物医学院,杨凌712100;中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验窀猪传染病研究室,哈尔滨150001 2. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验窀猪传染病研究室,哈尔滨150001 3. 西北农林科技大学动物医学院,杨凌,712100 |
| 基金项目： | 国家科技支撑计划（2006BAD06A03） |
| 摘 要： | 【目的】本研究旨在比较6种检测猪瘟病毒方法的优缺点。【方法】应用病毒分离、胶体金免疫层析试纸条、抗原捕捉ELISA、反转录-聚合酶链式反应(RT-PCR)、TaqMan荧光定量RT-PCR(RT-qPCR)和反转录-环介导等温扩增方法(RT-LAMP)等6种方法,分别对50份疑似猪瘟病料中的猪瘟病毒(Classical swine fevervirus,CSFV)进行检测。【结果】结果表明:RT-qPCR和RT-LAMP方法检出阳性样品数为13份,RT-PCR为11份,病毒分离为10份,抗原捕捉ELISA为9份,胶体金试纸条为8份;6种方法均检测为阳性8份,均为阴性37份。【结论】结果提示,在对猪瘟病毒进行检测时,RT-qPCR、RT-LAMP和RT-PCR由于其灵敏性高,可作为首选检测方法,但操作时需要避免假阳性的出现;病毒分离方法虽然操作繁琐,但结果准确,是确诊猪瘟必不可少的检测方法;抗原捕捉ELISA和胶体金试纸条检测时间较短,由于其敏感性较低所限,主要用于对畜群进行检测,不适合个体检测。 |
| 关 键 词： | 关键词：猪瘟病毒 检测方法 优缺点 |
| 收稿时间： | 2010/1/10 0:00:00 |
| 修稿时间： | 2010/3/15 0:00:00 |
Comparison of six detection methods for classical swine fever virus |
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| Xiangpeng Wang,Xingjuan Zhang,Yuan Sun,Na Li,Fan He,Tianming Chang,Hongyu Li,Zengqi Yang and Huaji Qiu.Comparison of six detection methods for classical swine fever virus[J].Acta Microbiologica Sinica,2010,50(8):1087-1093. | |
| Authors: | Xiangpeng Wang Xingjuan Zhang Yuan Sun Na Li Fan He Tianming Chang Hongyu Li Zengqi Yang and Huaji Qiu |
| Institution: | College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China;Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China |
| Abstract: | |
| Keywords: | Keywords: classical swine fever virus detection methods advantages and disadvantages |
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