一株马桑根瘤内生菌纯培养物的分类鉴定

从尼泊尔马桑(Coriaria nepalensis)根瘤中分离到一株内生菌纯培养物Cs146,它能使生长在半固体琼脂斜面和珍珠岩上的原寄主植物结瘤。该纯培养物具有Frankia属典型的形态和培养特征。在放线菌样的菌丝体上着生有泡囊和孢囊。在28～30℃下均能在液体和固体培养基上缓慢生长。菌丝呈橙黄色,产淡黄色可溶性色素,无气丝。但是,菌株Cs146的生理类型;细胞壁氨基酸组分和全细胞糖型等均与已知弗兰克氏菌有明显差别。因此认为,马桑根瘤内生菌Cs146应为Frankia类群中一个与其他弗兰克氏菌不同的新成员。

IDENTIFICATION OF A PURE CULTURE OF NODULAR ENDOPHYTE FROM CORIARIA NEPALENSIS

A Frankia-like strain, Cs146, was isolated from nodules of Coriaria nepalensis in southwest China. The isolate is an actinomycete with vesicles and sporangia borne on the hyphae, and shown to have an ability to nodulate the host plant. No aerial hyphae is formed, but the substrate hyphae are usually orange and produce soluble yellow pigment. Cs146 could grow quite slow at 28-30 degrees C on both liquid and solid media. However, the isolate is greatly different from other frankiae in physiology (intermediate physiologocal group between A and B trains), cell wall composition of characteristical amino acids (meso-DAP plus glycine) and whole cell sugar patterns (xylose plus little amount of galactose and arabinose). Cs146 thus appears to be a member of a distinct Frankia lineage.