苏云金芽孢杆菌不同亚种菌株制剂毒力效价的研究

采用苏云金芽孢杆菌制剂美国标准品:Bacillus thuringiensis subsp. Kurstaki HD-1-S-1980(H_(3a3b))对不同亚种制剂:Bacillus thuringiensis subsp. Dendrolimus U菌株(H_(4a4b))和Bacillus thuringiensis subsp. Galleriae C88菌株(H_5a5b))进行了毒力效价的研究。以U菌株制备的标样与美国标准品对不同试虫进行了比较。生物测定的结果表明,U菌株标样的效价分别为18666.6IU/mg(试虫为马尾松毛虫Dendrolimus punctatus 2龄幼虫)、22956.5IU/mg(试虫为小菜蛾Plutella xylostella 2龄幼虫),均高于美国标样16000IU/mg的水平。其敏感度为小菜蛾大于马尾松毛虫。以U菌株和C88菌株生产的中试产品用美国标准品进行毒力测定,试虫为马尾松毛虫,其结果表明,U菌株产品毒力效价为36444.4IU/mg;C88菌株产品为28521.7IU/mg,两者均明显高于美国标准品。试验结果还表明,采用美国标准品及马尾松毛虫和小菜蛾为…

STUDY ON THE TEST AND EVALUATION OF THE TOXICITY OF THE STRAINS PREPARATION OF THE DIFFERENT SUBSPECIES OF BACILLUS THURINGIENS1S AND ON THE RESEARCH INTO ITS STANDARDIZATION

In this paper using the preparation of Bacillus thuringiensis of USA standard sample-subsp. kurstaki HD-l-S-1980 (H3a3b) the toxicity to different subspecies preparations such as subsp. dendrolimus U strain(H4a4b), subsp. galleriae C88 strain (H5a5b) is tested and evaluated. And product's standardization is also researched. Comparing their toxicity to various test-insects between the standard sample prepared from U strain and USA, stamdard sample, the boilogical determined results are as follws: the toxicity of U strain standard sample is respectively 18666.6IU/mg(test-insects: Dendrolimus punctatus, 2nd larva), 22956.5IU/mg(test-insects: Plutella kylostella, 2nd larva). The above-mentioned toxicity evaluations are higher than that of 16000 IU / mg of USA standard sample. The comparison in susceptibility shows as follows: suscepticibility of plutella kylostella is stronger than that of Dendrolimus punctatus. The products from U strain and C88 strain are trial-produced in medium-scale. The product's toxicity to test-insect Dendrolimus punctatus is determined using USA standard sample. The results are obtained as follows the toxicity evaluation of U strain products is 36444.4 IU / mg; that of C88 strain products is 28521.7 IU / mg. The both are obviously higher than that of USA standard sample. This proves that different subspecies strain's toxicity to the same test-insect is with defference and it is an important way to raise product's toxicity by improving and optimizing process in production. The whole experiment shows it is feasible to rectify the evaluation of the toxicity of our country's present products using USA standard sample, Dendrolimus punctatus and Plutella kylostella as test-insets. And it is simple and convienient as well as save time and save effort to make statistical analysis in computer with Basic program. To do statistical and operational analysis using our designed basic, programming by micro-computer is more accurate and repid than by caculator.