抗鳗弧菌独特型单克隆抗体的制备及鉴定

利用具有中和活性的抗鳗弧菌单克隆抗体 4A6作为免疫原 ,通过单克隆抗体技术制备出 7株分泌抗独特型单抗的杂交瘤细胞。以ELISA竞争抑制实验及诱导Ab3的功能实验证实 ,其中 4株属于Ab2 β,有可能用于疫苗生产。

PRODUCTION AND DETECTION OF MONOCLONAL ANTI-IDIOTYPE ANTIBODIES AGAINST VIBRIO ANGUILLARUM

Vibrio anguillarum is the pathogenic bacteria of Vibriosis, which is an infectious disease found in various fish species. Seven monoclonal anti-idiotype antibodies(mAb2) were raised against mAb1 4A6. Identification of subgroup showed that 1H5, 1D1, 2B12 and 2F12 belonged to IgG2b, 2H12 and 1H12 to IgG2a and 1E10 to IgG3. The titers of these mAb2 ascites were 1 x 10(-4)-1 x 10(-6). The capacity of the mAb2 to inhibit the binding between mAb1 and antigen was investigated with the competitive inhibition ELISA. The results showed that 1D1, 1E10 1H5 and 2H12 mAb2 were able to inhibit this binding. Another experiment demonstrated that four mAb2(1D1, 1E10, 1H5 and 2H12) might induce Balb/c mice to produce Ab3 and these Ab3 competed the same antigen epitopes with Ab1. These results indicate that mAb2(1D1, 1E10, 1H5 and 2H12) are likely to represent internal image of antigen and belong to Ab2 beta. They might be employed to induce antibodies against pathogenic epitopes of V. anguillarum in vivo so as to gave the safe and effective vaccine.