一种产生纤溶酶的链霉菌C-3662的鉴定及发酵研究

对一株从土壤中分离的纤溶酶产生菌链霉菌C-3662的形态、培养、生理生化和化学分类特征进行了研究，发现其与泛温链霉菌(Streptomyces eurythermus Corbaz et al. 1968)的特征很相符。通过对链霉菌C-3662的16S rDNA序列进行测定与比对，发现它与泛温链霉菌的16S rDNA序列也有高达98.19%的同源性。根据多相分类原则，认为链霉菌C-3662属于泛温链霉菌。对链霉菌C-3662的发酵培养基组成等的研究表明，合适的发酵培养基中应含有氮源黄豆饼粉2.0%、碳源淀粉1.0%和葡萄糖2.5%以及少量的无机盐类如硫酸镁。链霉菌C3662的发酵过程研究表明，纤溶酶是在菌丝体生长停止之后才大量产生。

The Identification and Fermentation of a Streptomyces Strain Producing a Fibrinolytic Enzyme

The morphological, cultural, physiological and chemotaxonomical characteristics of Streptomyces C-3662, a soil isolate which produces a fibrinolytic enzyme, were studied. Most of these characteristics are the same or very similar to those of Streptomyces eurythermus. The 16S rDNA sequence of the strain was also determined and compared with those in the Genbank, resulting a mucleotide sequence identity of 98.19% with 16S rDNA of Streptomyces eurythermus. From the polyphasic taxonomical view, Streptomyces C-3662 belongs to Streptomyces eurythermus. Suitable medium for optimal fermentation of fibrinolytic enzyme by Streptomyces C-3662 comprises 2.5% glucose and 1.0% starch as carbon sources, 2% soybean meal as nitrogen source and some inorganic salts such as MgSO 4. The fermenttion course of Streptomyces C-3662 was investigated which showed that fibrinolytic enzyme was produced after the growth of Streptomyces C-3662 nearly ceased.