一种短杆状耐辐射菌的分离与鉴定

从北京地区公园湖岸土壤中分离到一株橙红色杆状耐辐射菌，细胞壁革兰氏染色为阴性，电镜显示菌体大小为06μm～16μm，略大于日本学者报道的Deinobacter grandis菌，过氧化氢酶的含量和分子量不同于D.radiodurans R1菌,分离菌的(G+C)mol%含量为707%, 16S rDNA序列分析表明，分离到的杆状耐辐射菌(RR5332)16S rRNA基因序列与Deinobacter grandis菌高度同源，提示RR5332归于Deinobacter菌属，并可能是该菌属中的一个新种。

Isolation and Characterization of a Novel Radiation-Resistant Rod-shaped Bacterium

A novel radiation-resistant bacterium was isolated from soil of lake bank in Beijing. The bacterium produced orange-pigmented colonies and formed rod-shaped cells that stained gram negative alike the Deinobacter grandis previously described by Japan's scientist. It was found with electron microscopy that the isolate is of 0.6 microm - 1.6 microm size and has a 30-40 nm thickness of cell wall, being slightly larger and thicker than the Deinobacter grandis. There was a difference in the concentration and molecule weight of catalase between the isolate and the Deincoccus radiodurans R1. The deoxyribonucleic acid guanine plus cytosine (G + C) base ratio was 70.7 mol%. 16S RNA gene sequencing also showed that this rod-shaped bacterium possessed a high homology with the Deinobacter grandis, suggesting that it might be classified into the genus Deinobacter and constitute a new species in this genus.