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藏东南地区土壤放线菌的生态分布及活性研究
引用本文:何建清,吴云锋,张格杰.藏东南地区土壤放线菌的生态分布及活性研究[J].微生物学报,2006,46(5):773-777.
作者姓名:何建清  吴云锋  张格杰
作者单位:1. 西北农林科技大学植保学院,陕西省农业分子生物学重点实验室,杨陵,712100;西藏大学农牧学院,林芝,860000
2. 西北农林科技大学植保学院,陕西省农业分子生物学重点实验室,杨陵,712100
3. 西藏大学农牧学院,林芝,860000
基金项目:国家自然科学基金(30571214),西北农林科技大学研究生教育创新计划(05YCH005)~~
摘    要:从藏东南原始森林、高山草甸、沼泽、粮田与保护地等不同植被、从2970~4590m不同海拔高度采集土样50份,用多种培养基分离中、低温放线菌,并对放线菌的数量、组成、生理生化特性以及它们的拮抗性等进行了研究。按放线菌的形态学特征进行了鉴定。结果表明:①从藏东南各种土壤中放线菌分离到9个属,其中束丝菌属在国内未见报道。以粮田中放线菌的数量和种类最多。②原始森林中拮抗性放线菌数量最多,提供了从原始森林土壤中可以筛选到更多拮抗性放线菌的重要信息。③抗革兰氏阳性细菌的放线菌菌株数较抗革兰氏阴性细菌的多,拮抗真菌的放线菌菌株数比拮抗细菌的多。④藏东南土壤链霉菌具有许多酶活性。

关 键 词:藏东南地区  放线菌分布  拮抗性
文章编号:0001-6209(2006)05-0773-05
收稿时间:2005-11-07
修稿时间:2005-11-072006-02-13

Activity and ecological distribution of actinomycetes from soil in the southeastern of Tibet
HE Jian-qing,WU Yun-feng,ZHANG Ge-jie.Activity and ecological distribution of actinomycetes from soil in the southeastern of Tibet[J].Acta Microbiologica Sinica,2006,46(5):773-777.
Authors:HE Jian-qing  WU Yun-feng  ZHANG Ge-jie
Institution:College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, China. hejq71@yahoo.com.cn
Abstract:The soil samples were collected from primeval forest, alpine meadow, marshy, grainfield and vegetable farmland ect. 9 different kinds of Vegetative type and from 2970 - 4590 altitude of southeastern of Tibet. The amount,composition, antimicrobial activity of mesophilic and psychrophilic actinomycetes and some mesophilic actinomycetes of physiological and biochemical characteristics were studied by normal methods. These actinomycetes were identified at genus level by their morphological properties. Results indicated that: (1) 9 genus of actinomycetes were isolated from soil and Actinosynnema was never reported in China. Grain field contains the largest quantity of actinomycetes. (2) Primeval forest contains the largest quantity of antimicrobal actinomycetes, so more and more antimicrobal actinomycetes information can be obtained from them. (3) The strains tested had stronger antimicrobial activity against Gram-positive bacteria than Gram-negative bacteria, had stronger antimicrobial activity against fungi than bacteria. (4) Many strains of streptomyces have some enzyme activities such as gelatin utilization, Milk-clotting enzyme etc.
Keywords:Southeastern of Tibet  Actinomycete ecological distribution  Antimicrobial activity
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