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紫杉醇高产菌株的原生质体诱变选育及其遗传变异初探
引用本文:赵凯,平文祥,马玺,刘军,周东坡.紫杉醇高产菌株的原生质体诱变选育及其遗传变异初探[J].微生物学报,2005,45(3):355-358.
作者姓名:赵凯  平文祥  马玺  刘军  周东坡
作者单位:1. 黑龙江大学生命科学学院,哈尔滨,150080;中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,哈尔滨,150001
2. 黑龙江大学生命科学学院,哈尔滨,150080
基金项目:黑龙江省“十五”重大攻关项目 (GA0 2C10 1),哈尔滨市重点项目 ( 0 114 2 112 6)~~
摘    要:以紫杉醇产生菌NCEU_1为出发菌株,分别采用紫外线和紫外线与氯化锂复合诱变方法对其原生质体进行诱变,获得了两株高产紫杉醇的突变株———UV4 0 - 1 9和UL50 - 6 ,其紫杉醇产量从出发菌株的314 0 7μg L分别提高至376 38μg L和392 6 3μg L ;同时,又采用RAPD和同工酶技术对出发菌株NCEU_1与两高产株UV4 0 - 1 9和UL50 - 6 间的遗传差异进行了研究。结果表明,出发菌株与诱变菌株之间以及两诱变菌株之间都存在明显差异,为进一步研究与紫杉醇合成相关基因及诱变株产量提高的分子机制奠定了基础

关 键 词:紫杉醇产生菌原生质体诱变遗传差异
文章编号:0001-6209(2005)03-0355-04
修稿时间:2004年8月17日

Breeding of high-yield strain of taxol by mutagensis of protoplast and primary discussion of genetic differences between mutants and their parent strain
ZHAO Kai,PING Wen-xiang,MA Xi,LIU Jun,ZHOU Dong-po.Breeding of high-yield strain of taxol by mutagensis of protoplast and primary discussion of genetic differences between mutants and their parent strain[J].Acta Microbiologica Sinica,2005,45(3):355-358.
Authors:ZHAO Kai  PING Wen-xiang  MA Xi  LIU Jun  ZHOU Dong-po
Institution:Life and Science college of Hei Longjiang University, Harbin 150080, China. zk395@yahoo.com.cn
Abstract:The breeding of high-yield strain of taxol was performed by protoplast mutagenesis of strain NCEU-1 using ultraviolet radiation and combined treatment of UV and LiCl. The mutants UV40-19 and UL50-6 were obtained, which raised the taxol yield from 314.07 microg/L to 376.38 microg/L and 392.63 microg/L respectively. Genetic differences between the mutants UV40-19, UL50-6 and their parent strain were primarily compared through random amplified polymorphic DNA (RAPD) and isozyme technique. The results showed that the genetic differences were very obviously between the parent strain and its mutants and between the two mutants, which laid foundation of molecular mechanism for the study of genes related to the taxol biosynthesis and mutants for raising the taxol yield.
Keywords:Taxol-producing fungi  Protoplast mutagensis  Genetic difference
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