| 1, 3-丙二醇发酵过程中盐浓度的胁迫作用 |
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| 引用本文： | 徐佳杰,刘朋波,陶春平,程骋,付水林,宫衡.1, 3-丙二醇发酵过程中盐浓度的胁迫作用[J].微生物学报,2008,24(6):1098-1102. |
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| 作者姓名： | 徐佳杰 刘朋波 陶春平 程骋 付水林 宫衡 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室, 上海 200237;华东理工大学生物反应器工程国家重点实验室, 上海 200237;华东理工大学生物反应器工程国家重点实验室, 上海 200237;华东理工大学生物反应器工程国家重点实验室, 上海 200237;华东理工大学生物反应器工程国家重点实验室, 上海 200237;华东理工大学生物反应器工程国家重点实验室, 上海 200237 |
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| 摘 要： | 通过对克雷伯氏菌(Klebsiella pneumoniae)甘油发酵生产1, 3-丙二醇(1, 3-PD)过程的研究发现, 盐浓度对 1, 3-PD发酵有胁迫作用。盐浓度较低时, 菌体生长和产物生成均维持较高速率; 盐浓度较高时会导致菌体生长减慢, 1, 3-PD最终浓度, 甘油到1, 3-丙二醇的转化率降低, 同时1, 3-丙二醇氧化还原酶受到抑制。在5 m3罐中控制合适的盐浓度可以提高1, 3-PD的发酵水平, 使1, 3-PD的最终浓度达到64 g/L, 转化率61%, 生产强度2.1 g/(L·h)。
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| 关 键 词： | 盐浓度 中试实验 发酵条件 氧化还原电位 |
1, 3-Propanediol Production Under Salt Stress |
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| Jiajie Xu,Pengbo Liu,Chunping Tao,Cheng Cheng,Shuilin Fu and Heng Gong.1, 3-Propanediol Production Under Salt Stress[J].Acta Microbiologica Sinica,2008,24(6):1098-1102. |
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| Authors: | Jiajie Xu Pengbo Liu Chunping Tao Cheng Cheng Shuilin Fu and Heng Gong |
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| Institution: | State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China;State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China |
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| Abstract: | Through studying the process of glycerol fermentation to 1, 3-propanediol(1, 3-PD) by Klebsiella pneumoniae, it was found that the cell growth and product (or by-product) production were under salt stress. Cell growth and product formation kept high rate at low salt concentration. High salt concentration led to low growth of cells, final concentration of 1, 3-PD and conversion from glycerol to 1, 3-PD, and, 1, 3-propanediol oxidoreductase activity decreased. When the salt concentration in 5 m3 bioreactor was controlled under appropriate manner, the concentration of 1, 3-PD production was markedly enhanced. The final 1, 3-PD concentration ,the conversion of glycerol to 1, 3-PD and productivity were 64 g/L, 61% and 2.1 g/(L·h). |
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| Keywords: | salt concentration middle industrial scale fermentation conditions oxidation reduction potential |
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