担子菌组成型漆酶产生特性的研究

运用平板筛选法从20株不同来源的菌株中得到一株产组成型漆酶的担子菌(Basidiomycete sp.)。它在自制DC培养基上产酶能力达到058IU/mL；振荡培养的产酶效果约是静置培养的4倍。漆酶的产生与生物量在培养8d内呈现平行关系，但随后酶量并不随之而继续上升。4g/L的酪氨酸和胰蛋白胨有利于酶的产生，分别可使酶活力最高增加到249IU/mL和20IU/mL。某些化学试剂对酶的产量尚未达到诱导效果，但丁香醛连氮和邻联甲苯胺对酶产量有一定的促进作用。40℃下刺激1h后，可使酶活力提高13倍。

PRDODUCING CHARACIERIZATION OF CONSTTUTIVE-FORM LACCASE BY BASIDIOMYCETE

A constitutive-laccase-producing fungus was gained from 20 strains of different sources with plate method. Laccase activity reached 0.58 IU/mL when the strain grew on DC medium with shaking cultivation, which was superior to stationary method by 4 times. The enzyme production paralleled with biomass in early 8 days, subsequently activities declined slightly though its biomass continued upward. Tyrosine and tryptone at the concentration of 4 g/L improved the enzyme production, reaching 2.49 and 2.0 IU/mL respectively. Some tested chemical agents seemed not to contribute to the increase of the enzyme production effectively except for syringaldazine and o-tolidine. Heatshock at 40 degrees C for 1 h made 1.3 times more enzyme expression.