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二元酸发酵过程中流加H2O2对细胞色素P450酶及产物生成的影响
引用本文:李书良,华玉涛,黄英明,焦鹏,曹竹安.二元酸发酵过程中流加H2O2对细胞色素P450酶及产物生成的影响[J].微生物学报,2002,42(3):359-363.
作者姓名:李书良  华玉涛  黄英明  焦鹏  曹竹安
作者单位:清华大学化工系生物化工研究所,北京,100084
基金项目:国家自然科学基金重点项目 ( 2 0 0 36 0 1 0 ),国家自然科学基金 ( 2 9976 0 2 2 )~~
摘    要:在二元酸发酵过程中流加H2O2对热带假丝酵母发酵生产二元酸有明显的促进作用,2mmol/L的H2O2对产酸的促进作用最为明显,比对照提高了26%。对细胞色素P450酶的分析表明,流加H2O2对细胞色素P450酶的活性有明显的促进作用,并且细胞色素P450酶的活性跟产酸成正相关。此外,还进一步分析了流加H2O2对产酸的促进机理。

关 键 词:热带假丝酵母,  长链二元酸,  H2O2,  细胞色素P450酶
文章编号:0001-6209(2002)03-0359-05

Effects of H2 O2 Addition on Cell Growth and Product Formation in Long-chain Dicarboxylic Acid Fermentation
Li Shuliang Hua Yutao Huang Yingming Jiao Peng Cao Zhu''''an.Effects of H2 O2 Addition on Cell Growth and Product Formation in Long-chain Dicarboxylic Acid Fermentation[J].Acta Microbiologica Sinica,2002,42(3):359-363.
Authors:Li Shuliang Hua Yutao Huang Yingming Jiao Peng Cao Zhu'an
Institution:Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, Beijing 100084, China.
Abstract:When Candida tropicalis was cultivated in shakig-flask with the H 2O 2 addition, DCA (Dicarboxylic Acid,DCA) concentration was increased, especially at 2mmol/L H 2O 2 concentration. The cytochrome P450 activity assays indicated that H 2O 2 addition significantly increased the activities of cytochrome P450 and DCA production positively correlated with the activities of cytochrome P450. The study on the cell growth demonstrated that the H 2O 2 addition inhibited the cell growth rate. However, the retarding effect was not irreversible since the cell growth rate could recover slowly to the original level after the H 2O 2 addition was halted. The mechanism of inducement on cytochrome P450 by H 2O 2 addition was also discussed in this article.
Keywords:Candida tropicalis  Long chain dicarboxylic acid  Hydrogen peroxide(H  2O  2)  Cytochrome P450
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