| 香菇135菌株特异SCAR标记在其原生质体单核中的分布 |
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| 引用本文： | 张美彦,谭琦,陈明杰,潘迎捷.香菇135菌株特异SCAR标记在其原生质体单核中的分布[J].微生物学报,2008,27(2):252-257. |
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| 作者姓名： | 张美彦 谭琦 陈明杰 潘迎捷 |
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| 作者单位： | 南京农业大学生命科学学院 南京 210095, 上海农业科学院食用菌研究所 上海 201106;上海农业科学院食用菌研究所 上海 201106;上海农业科学院食用菌研究所 上海 201106;上海水产大学 上海 200090 |
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| 基金项目： | 上海市农委资助项目（项目编号：沪农科攻字（2004）第2-16号） |
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| 摘 要： | 以香菇保藏菌种庆元135以及庆元出菇的135新鲜子实体组织分离得到的菌丝为材料制备原生质体，分别获得58和83个原生质体单核体；经交配型鉴定后，用已获得的135特异SCAR引物对单核进行PCR扩增，统计SCAR条带的分布。结果显示：原生质体单核体分为A1B1和A2B2两种交配型，而特异条带仅存在于后者中，证明135特异SCAR标记是稳定遗传的，也为鉴定以带标记核为亲本之一的杂交后代提供了依据，这就进一步拓宽了分子标记应用于菌株鉴定的适用范围。
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| 关 键 词： | 香菇育种，分子标记，遗传规律 |
Distribution of a specific SCAR marker among Lentinula edodes protoplast monokaryons for strain 135 |
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| ZHANG Mei-Yan,TAN Qi,CHEN Ming-Jie and PAN Ying-Jie.Distribution of a specific SCAR marker among Lentinula edodes protoplast monokaryons for strain 135[J].Acta Microbiologica Sinica,2008,27(2):252-257. |
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| Authors: | ZHANG Mei-Yan TAN Qi CHEN Ming-Jie and PAN Ying-Jie |
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| Institution: | College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China;Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China;Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China;Shanghai Fisheries University, Shanghai 200090, China |
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| Abstract: | Refrigerated mycelium and fresh fruitbody tissue-isolated mycelium of Lentinula edodes strain 135 were used to prepare protoplast monokaryons. Fifty-eight and 83 monokaryons were isolated respectively and their mating types were determined. PCR amplification employing specific primers was used to establish the distribution among the protoplast monokaryons of a SCAR marker specific for strain 135. Protoplast monokaryons segregated into either A1B1 or A2B2 mating type, and the SCAR marker was detected only in the latter. The outcome is a regular distribution pattern that demonstrates the stable inheritance of the SCAR marker and is fundamental to identification of offspring arising from crosses between protoplast monokaryons of this particular mating type and other karyons. |
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| Keywords: | breeding of Lentinula edodes molecular marker inheritance pattern |
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