首页 | 本学科首页   官方微博 | 高级检索  
   检索      

产甾体皂甙华重楼内生菌的筛选与鉴定
引用本文:赵明,贺声蓉,陈小静,黄春萍,王一丁.产甾体皂甙华重楼内生菌的筛选与鉴定[J].微生物学报,2005,45(5):776-779.
作者姓名:赵明  贺声蓉  陈小静  黄春萍  王一丁
作者单位:1. 四川师范大学生命科学学院,成都,610068
2. 四川师范大学生命科学学院成都610068
基金项目:四川师范大学校级科研基金“团队项目”~~
摘    要:从华重楼(Paris polyphyllavar.chinensisFranch)的地下块茎中分离筛选得到2株可能产生甾体皂甙的内生菌(SS01和SS02),薄层层析检测菌株SS01、SS02的发酵产物分别有3条和2条层析带与重楼总皂甙的层析带迁移率相当。形态和生理生化特征初步表明SS01和SS02分属于肠杆菌科(Enterobacteriaceae)和芽孢杆菌属(Bacillussp.)细菌。扩增、测序得到SS01和SS02的部分16S rDNA序列,GenBank接收号分别为AY842143和AY842144。用Blastn调出与菌株16SrDNA同源的序列,用Clustal W进行多重序列对比,用软件Phylip按Neighbor-Joining方法构建16SrDNA系统发育树。菌株SS01和SS02分别与Cedecea davisae DSM4568、Paenibacillus daejeonensis处于同一分支,相似性分别为98.9%和97.7%,将它们鉴定为Cedecea davisae SS01和Paenibacillus daejeonensis SS02。

关 键 词:华重楼  内生菌  甾体皂甙  16SrDNA序列分析
文章编号:0001-6209(2005)05-0776-04
收稿时间:2004-12-27
修稿时间:2004年12月27

Screening and identification of steroidal saponins-producing endophytes from Paris polyphylla var. Chinensis Franch
ZHAO Ming,HE Sheng-rong,CHEN Xiao-jing,HUANG Chun-ping,WANG Yi-ding.Screening and identification of steroidal saponins-producing endophytes from Paris polyphylla var. Chinensis Franch[J].Acta Microbiologica Sinica,2005,45(5):776-779.
Authors:ZHAO Ming  HE Sheng-rong  CHEN Xiao-jing  HUANG Chun-ping  WANG Yi-ding
Institution:College of Life Sciences, Sichuan Normal University, Chengdu 610068, China
Abstract:Two endophytic strains SS01 and SS02 with the potential for producing steroidal saponins were isolated from the underground stems of Paris polyphylla var. chinensis Franch. The TLC comparison indicated that there are 3 sports with similar R(f) between the metabolites of SS01and the saponins of Paris polyphylla var. chinensis Franch. And there are 2 sports with similar R(f) between the metabolites of SS02 and the saponins of Paris polyphylla var. chinensis Franch. The characteristics of morphology, physiological and biochemical showed that SS01 belonged to Enterobacteriaceae and SS02 belonged to Bacillus sp.. The 16S rDNA of SS01 and SS02 were PCR and sequenced. The accessions of GenBank are AY842143 and AY842144, respectively. The two 16S rDNA phylogenetic trees were constructed by comparing with the published 16S rDNA sequences of the relative bacteria species. In the first phylogenetic tree SS01 and Cedecea davisae DSM 4568 was the closest relative with 98.9% sequence similarity,and in the second phylogenetic tree SS02 and Paenibacillus daejeonensis was the closest relative with 97.7% sequence similarity. According to the phylogenetic analysis they were identified as Cedecea davisae SS01 and Paenibacillus daejeonensis SS02, respectively.
Keywords:Paris polyphylla var  chinensis Franch  Endophyte  Steroidal saponins  16S rDNA sequence analysis
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《微生物学报》浏览原始摘要信息
点击此处可从《微生物学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号