禾谷镰孢菌β-微管蛋白基因克隆及其与多菌灵抗药性关系的分析

应用3对引物，从禾谷镰孢菌(Gibberella zeae)对多菌灵(MBC)的敏感菌株(MBC^R)和田间及室内诱导抗药性菌株(MBC^R)中扩增β-微管蛋白基因。该基因全长1631bp，包含3个内含子，编码447aa，与其他常见植物病原丝状真菌β-微管蛋白基因的氨基酸同源性达95．12％～99．30％。MBC^R和MBC^R菌株核苷酸序列分析表明，MBCR菌株未发生任何位点的突变，说明G．zeae对MBC的抗药性机制并非像其他丝状真菌一样由β-微管蛋白198位氨基酸突变所致。

Cloning of β-tubulin Gene from Gibberella zeae and Analysis its Relationship with Carbendazim-resistance

Whole beta-tubulin genes from wild carbendazim(MBC)-sensitive isolate, field MBC-resistant isolate and induced MBC-resistant mutant of Gibberella zeae were cloned and sequenced with 3 pairs of primers. These genes have 1631bp length, including 3 introns, encoding 447 amino acid. The homology of amino acid tubulin gene of G. zeae with that of other common plant pathogenic filamentous fungi is from 95.12% approximately 99.30%. Sequence comparison among MBCs, field MBCR and induced MBCR isolates revealed there was no mutation, even one. So it can be concluded that the mechanism of MBC-resistance to G. zeae is different from other filamentous fungi caused by point mutation at amino acid position 198 or other position of beta-tubulin gene.