烟碱降解细菌的分离、鉴定及其降解性能的初步研究北大核心

从福建三明地区的土壤中分离得到一株能够高效降解烟碱的菌株 ,编号为DN2。该菌经常规的形态、生理生化分析以及 16SrDNA序列同源性分析 ,鉴定为Ochrobactrumintermedium ,属于α_变形杆细菌纲。该菌在 30℃～4 0℃和pH 6 . 0～ 9. 0范围内具有较高的降解活性 ,其最适值分别为 30℃和 6 5 ,烟碱的耐受浓度在无机盐培养基中可达到 4 0 0 0mg L。该菌能够以烟碱为唯一碳源生长 ,对于 5 0 0mg L烟碱的降解速率为 15mg L·h ,36h烟碱降解率为97. 6 5 %。该菌在烟草工业和环境保护上可能具有应用前景。

Isolation and identification of a strain DN2 degrading nicotine

A bacterial strain which was able to utilize nicotine as its sole carbon source was isolated from soil in which tobacco had grown at Sanming region in FUjian Province and named as DN2. Upon chemotaxonomic characterization and phylogenetic inference based 16S rDNA analysis, the strain DN2 was identified as a-proteobacteria, Ochrobactrum intermedium. For DN2 degrading nicotine, the optimal pH and temperature is 6.5, 30 degrees C respectively. It can tolerate high-concentration of nicotine up to 4000 mg/L in basal media. Using 500 mg/L nicotine as its sole carbon, the strain was able to degrade 15 mg/L of nicotine per liter per hour and reached its stationary phase in about 36 hours.