T90-1木霉菌的筛选和对草莓灰霉病菌作用机制的研究

木霉菌（Trichoderma）作为一种重要的生防因子，可以产生几丁质酶降解植物的多种病原真菌细胞壁。利用低能离子束注入木霉菌使其产生变异，再通过初筛选和复筛选两个过程，获得T90_1木霉菌株，并用草莓灰霉病菌（Botrytis cinerea Pers.）来检验T90_1防治真菌病害的能力。发现该菌株通过侵染、缠绕等多种重寄生方式，并分泌降解病原真菌细胞壁的物质，使病原菌原生质外渗，改变细胞内有序的代谢状况，从而抑制或杀死病原菌。初步揭示该菌株抗真菌的相关机制。

T90-1 Screening Progress and Its Application for Control of Botrytis cinerea Pers.

Trichoderma spp. are promising biocontrol agents (BCAs) of plant diseases. Trichoderma can decompose cell wall of some plant pathogenic fungus through secreting chitinolytic enzymes. Trichoderma were implanted by ion to produce aberrance. Whereafter first screened 12 aberrance of Trichoderma strains through eyeballing. And then screened 12 aberrances and CK through four ways again. T90_1, author was selected from these aberrance strains and CK. Further experiment demonstrated that T90_1 can hyperparasitize Botrytis cinerea Pers. through enwind, infection, attachment filament etc. Zymolytic liquid of T90_1 can decompose mycelial wall that account for T90_1 excreteing chitinolytic enzymes during zymolysis.