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云芝Trametes versicolor1126所产漆酶对靛蓝废水脱色的初步研究
引用本文:闫世梁,李培睿,李宗义,李宗伟,秦广雍.云芝Trametes versicolor1126所产漆酶对靛蓝废水脱色的初步研究[J].微生物学报,2008,27(2):309-315.
作者姓名:闫世梁  李培睿  李宗义  李宗伟  秦广雍
作者单位:郑州大学离子束生物工程省重点实验室 郑州 450052;郑州大学离子束生物工程省重点实验室 郑州 450052; 河南师范大学生命科学学院 新乡 453007;郑州大学离子束生物工程省重点实验室 郑州 450052; 河南师范大学生命科学学院 新乡 453007;郑州大学离子束生物工程省重点实验室 郑州 450052;郑州大学离子束生物工程省重点实验室 郑州 450052
基金项目:国家重点基础研究发展规划973项目(No. 2004CB719604)
摘    要:考察了云芝Trametes versicolor 1126发酵培养中漆酶酶活和pH的变化,同时研究了羧甲基纤维素钠及苯酚添加量对漆酶活力的影响。结果表明当培养基中加入0.8%羧甲基纤维素钠、100mg/L苯酚时,均能明显提高漆酶的活力。以漆酶/HBT介质体系对靛蓝废水进行脱色,反应100min后,脱色率达90.8%。使用漆酶处理靛蓝废水具有广阔的应用前景

关 键 词:大型真菌,纺织染料,降解

Preliminary study on decolorization of indigo dye wastewater with laccase from Trametes versicolor 1126
YAN Shi-Liang,LI Pei-Rui,LI Zong-Yi,LI Zong-Wei and QIN Guang-Yong.Preliminary study on decolorization of indigo dye wastewater with laccase from Trametes versicolor 1126[J].Acta Microbiologica Sinica,2008,27(2):309-315.
Authors:YAN Shi-Liang  LI Pei-Rui  LI Zong-Yi  LI Zong-Wei and QIN Guang-Yong
Institution:Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China;Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China; College of Life Sciences, Henan Normal University, Xinxiang 453007, China;Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China; College of Life Sciences, Henan Normal University, Xinxiang 453007, China;Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China;Provincial Key Laboratory of Ion Beam Bioengineering, Zhengzhou University, Zhengzhou 450052, China
Abstract:The dynamic variation of laccase activity and pH were detected during fermentation of Trametes versicolor 1126. The effects of sodium carboxymethyl cellulose and phenol on laccase activity were also studied. The results indicated that 0.8% sodium carboxymethyl cellulose and 100mg/L phenol in the medium can enhance the activity of laccase obviously. Indigo dye wastewater was degraded in laccase/HBT mediator system. The decolorization rate reaches 90.8% in 100 minutes. A practical way using laccase to decolorize indigo dye wastewater has extensive prospect.
Keywords:larger fungi  textile dye  degradation
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