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| 鸭肝炎病毒新血清型基因组序列分析 | |
| 引用本文： | 施少华,程龙飞,傅光华,陈红梅,陈珍,杨维星,苏敬良,黄瑜.鸭肝炎病毒新血清型基因组序列分析[J].微生物学报,2009,49(3):309-315. |
| 作者姓名： | 施少华 程龙飞 傅光华 陈红梅 陈珍 杨维星 苏敬良 黄瑜 |
| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福州,350013;福建省畜禽疫病防治工程技术研究中心,福州,350013 2. 福建省农业科学院畜牧兽医研究所,福州,350013 3. 江西农业大学动物科学技术学院,南昌,330045 4. 中国农业大学动物医学院,北京,100094 |
| 摘 要： | 目的]为了揭示鸭肝炎病毒新血清型(DHV-N)G株的演化及其与DHV-1的基因序列差异.方法]运用RT-PCR结合5'-/3'-RACE策略对DHV-N G株基因组进行扩增,克隆测序后进行序列分析.结果]DHV-NG株全基因组序列除12 nt poly(A)尾外全长共7774 nt,含有一个大的开放阅读框,编码2251 aa的蛋白前体,其基因组结构:5'UTR-L-VP0-VP3-VP1-2A1-2A2-2B-2C-3A-3B-3C-3D-3'UTR;3'UTR长为366 nt,比1型鸭肝炎病毒C80株多52 nt;VP1蛋白与DHV-1相比发生较大的变异,特别在其第140～221位;其基因组与DHV-1、韩国DHV-N和台湾DHV-N的同源率分别为72.8%～73.4%、96.3%～96.5%和78.3%.结论]DHV-N G株基因组结构与DHV-1存在明显差异,在DHV-N成员DHV-N G株与韩国DHV-N关系更为密切. |
| 关 键 词： | 鸭肝炎病毒 新血清型 序列分析 |
| 收稿时间： | 2008/10/16 0:00:00 |
| 修稿时间： | 2008/12/11 0:00:00 |
Genomic sequence of a new serotype duck hepatitis virus |
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| Shaohua Shi,Longfei Cheng,Guanghua Fu,Hongmei Chen,Zhen Chen,Weixing Yang,Jingliang Su and Yu Huang.Genomic sequence of a new serotype duck hepatitis virus[J].Acta Microbiologica Sinica,2009,49(3):309-315. | |
| Authors: | Shaohua Shi Longfei Cheng Guanghua Fu Hongmei Chen Zhen Chen Weixing Yang Jingliang Su and Yu Huang |
| Institution: | Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;College of animal science and technology, Jiangxi Agricultural University , Nanchang 330045, China;College of Veterinary Medicine, China Agricultural University, Beijing 100094, China;Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China |
| Abstract: | |
| Keywords: | Keywords: duck hepatitis virus new serotype sequence analysis |
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