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防治烟草赤星病有益内生细菌的筛选及抑菌作用
引用本文:易龙,肖崇刚,马冠华,王万能,龙良鲲.防治烟草赤星病有益内生细菌的筛选及抑菌作用[J].微生物学报,2004,44(1):19-22.
作者姓名:易龙  肖崇刚  马冠华  王万能  龙良鲲
作者单位:西南农业大学植物保护学院,重庆,400716
基金项目:重庆市教委科学技术研究项目 ( 1180 7)~~
摘    要:从健康烟草的叶、茎中分离到302株非病原内生细菌,通过平板对峙培养,筛选出对烟草赤星病菌[Alternaria alternata(Fr.)Keissl]不同致病力的4个代表菌株均有拮抗作用的11个菌株。室内测定其对赤星病菌抑菌带的宽度达5.5~13.2mm;拮抗、防病试验测定,来自叶片内的内生菌株Itb162对赤星病菌有较强和稳定的拮抗作用,对赤星病有52.0%的防病效果。无菌滤液实验表明,拮抗内生细菌Itb162无菌滤液在一定浓度范围内均能有效地抑制菌丝生长,减少孢子萌发,且浓度越高,抑制能力越强。

关 键 词:烟草赤星病菌,  内生细菌,  筛选,  生物防治
文章编号:0001-6209(2004)01-0019-04
修稿时间:2003年4月21日

Inhibition and Screening of Beneficial Endophytic Bacteria to Control Tobacco Brown Spot
YI Long,XIAO Chong_Gang,MA Guan_Hua WANG Wan_Neng LONG Liang_Kun.Inhibition and Screening of Beneficial Endophytic Bacteria to Control Tobacco Brown Spot[J].Acta Microbiologica Sinica,2004,44(1):19-22.
Authors:YI Long  XIAO Chong_Gang  MA Guan_Hua WANG Wan_Neng LONG Liang_Kun
Institution:YI Long XIAO Chong_Gang * MA Guan_Hua WANG Wan_Neng LONG Liang_Kun
Abstract:bacterial strains were isolated from inner of tobacco leaf . It was found that by pairing culture on PDA, 11 potential biocontrol isolates showed good antagonistic effects on 4 strains of different virulent Alternaria alternata, the pathogen of tobacco brown spot, and the inhibition zone diameter ranged from 5 5mm to 13 2mm. The result from experiment trial at greenhouse and pairing culture showed the effectiveness of bacterial preparations from Itb162 on reduction the incidence of tobacco brown spot. The control efficiency reached 52 0%. The results from culture filtrate showed that Itb162 was effective for inhibiting mycelium growth and reducing spore germination of A.alternata in certain of concentration.
Keywords:Alternaria alternata    Endophytic bacterium  Screening  Biocontrol
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