| 甲醛致酵母菌与大肠杆菌DNA-蛋白质交联作用的比较 |
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| 引用本文： | 甘耀坤,徐钱,彭光银,吴凯,刘凯于.甲醛致酵母菌与大肠杆菌DNA-蛋白质交联作用的比较[J].微生物学报,2007,47(1):164-167. |
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| 作者姓名： | 甘耀坤 徐钱 彭光银 吴凯 刘凯于 |
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| 作者单位： | 1. 广西玉林师范学院化学与生物系,玉林,537000
2. 华中师范大学生命科学学院环境科学实验室,武汉,430079 |
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| 基金项目： | 国家自然科学基金;湖北省科技攻关项目 |
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| 摘 要： | 为了探讨甲醛致真核生物与原核生物DNA-蛋白质交联(DNA-protein crosslinks,DPC)的作用,以毕赤酵母(Pichia pastoris)和大肠杆菌(Escherichia coli)DH5α为材料,采用KCl-SDS沉淀法检测液态甲醛染毒后酵母菌与大肠杆菌中DPC含量。结果表明,低浓度(25μmol/L)甲醛不能引起酵母菌和大肠杆菌的DPC(P>0.05),而较高浓度(125和625μmol/L)甲醛可引起酵母菌和大肠杆菌明显的DPC(P<0.05);另外,酵母菌的DPC系数是大肠杆菌的DPC系数的10倍左右。这表明甲醛致真核细胞和原核细胞的DPC作用具有剂量效应关系,而且真核细胞的DPC系数比原核细胞的DPC系数更高。
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| 关 键 词： | 甲醛 毕赤酵母 大肠杆菌 |
| 文章编号： | 0001-6209（2007）01-0164-04 |
| 收稿时间： | 2006/6/27 0:00:00 |
| 修稿时间： | 2006-06-27 |
A Comparison between the effects on DNA-protein cross-links of Saccharomyces and E.coli induced by formaldehyde |
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| GAN Yaokun,XU Qian,PENG Guangyin,WU Kai and LIU Kai-yu.A Comparison between the effects on DNA-protein cross-links of Saccharomyces and E.coli induced by formaldehyde[J].Acta Microbiologica Sinica,2007,47(1):164-167. |
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| Authors: | GAN Yaokun XU Qian PENG Guangyin WU Kai and LIU Kai-yu |
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| Institution: | Department of Chemistry and Life Science; Yulin Normal College of Gangxi; Yulin 537000; China;College of Life Science; Central China Normal University; Wuhan 430079; China;College of Life Science; Central China Normal University; Wuhan 430079; China;College of Life Science; Central China Normal University; Wuhan 430079; China;College of Life Science; Central China Normal University; Wuhan 430079; China |
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| Abstract: | To explore the effect of formaldehyde on DNA-protein cross-links (DPC) in eucaryotic cells and prokaryotic cells, Pichia pastoris and E. coli DH5alpha were chosen as materials to evaluate the amount of DPC induced by liquid formaldehyde in vivo by the method of KCl-SDS assay. The results showed that formaldehyde could not induce DPC at low dose(25micromol/L,P > 0.05), but could obviously induce DPC at higher dose (125 and 625micromol/L, P < 0.05). The DPC coefficient in Pichia pastoris was 10-fold higher than that in E. coli DH5alpha. It is concluded that formaldehyde could induce DPC in eucaryotic cells and prokaryotic cells with a dose-dependent manner and the DPC coefficient in Pichia pastoris is higher than that in E. coli DH5alpha. |
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| Keywords: | DPC |
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