| 黑曲霉木聚糖酶在工业酒精酵母中的分泌表达 |
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| 引用本文： | 李海燕,祝令香,毛爱军,董志扬.黑曲霉木聚糖酶在工业酒精酵母中的分泌表达[J].微生物学报,2005,45(1):135-138. |
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| 作者姓名： | 李海燕 祝令香 毛爱军 董志扬 |
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| 作者单位： | 中国科学院微生物研究所,微生物资源前期开发国家重点实验室,北京,100080 |
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| 基金项目： | 国家“8 63计划”( 2 0 0 1AA2 14 15 1)，国家自然科学基金面上项目 ( 3 0 170 2 18)~~ |
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| 摘 要： | 用重叠延伸PCR方法从黑曲霉 (Aspergillusniger)UV 11的基因组DNA中克隆出木聚糖酶的cDNA基因 ,构建了由酵母乙醇脱氢酶 (ADH1)启动子和终止子引导表达、木聚糖酶自身信号肽引导分泌、rDNA序列介导的酵母整合型分泌表达质粒pAX2。用pAX2与酵母YEp型G4 18抗性质粒共转化野生型工业酒精酵母S .cerevisiae 2 346 ,获得了整合型分泌表达木聚糖酶的酵母重组菌株XY2。发酵分析表明该工程菌能够明显提高酒精生产率
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| 关 键 词： | 木聚糖酶 整合分泌表达 酒精酵母工程菌 酒精生产率 |
| 文章编号： | 0001-6209(2005)01-0135-04 |
| 修稿时间： | 2004年5月9日 |
Integrative expression of XynB of Aspergillus niger UV-11 in industrial yeast |
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| LI Hai-yan,ZHU Ling-xiang,MAO Ai-jun,DONG Zhi-yang.Integrative expression of XynB of Aspergillus niger UV-11 in industrial yeast[J].Acta Microbiologica Sinica,2005,45(1):135-138. |
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| Authors: | LI Hai-yan ZHU Ling-xiang MAO Ai-jun DONG Zhi-yang |
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| Institution: | State Key Laboratory of Microbio Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. lhyyy205@sohu.com |
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| Abstract: | The cDNA sequence of beta-xylanase gene (xynB) was cloned from Aspergillus niger UV-11. It was inserted into the yeast expression vector and the recombinant plasmid pAX2 was obtained. The plasmid pAX2 was introduced into an industrial S. cerevisiae 2.346 and integrated into yeast genome by co-transformation of a YEPtype plasmid pBEJ16 carrying G418 resistance. The stable engineered yeast strain XY2 was obtained. It could express and secret extracellular xylanase, and enhance the alcohol production in wheat flour fermentation compared with the host strain S. cerevisiae 2.346. |
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| Keywords: | xylanase Integrative expression Industrial yeast Alcohol production |
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