| 猪流行性腹泻病毒核衣壳蛋白与感染细胞核磷蛋白的共定位分析 |
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| 引用本文： | 吕茂杰,陈建飞,时洪艳,陈小金,范秀萍,申识川,冯力?.猪流行性腹泻病毒核衣壳蛋白与感染细胞核磷蛋白的共定位分析[J].微生物学报,2011,51(5):643-647. |
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| 作者姓名： | 吕茂杰 陈建飞 时洪艳 陈小金 范秀萍 申识川 冯力? |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室猪传染病研究室,哈尔滨,150001 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费(200805) |
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| 摘 要： | 【目的】阐明猪流行性腹泻病毒(PEDV)核衣壳蛋白与病毒感染细胞核仁成分B23.1蛋白的共定位特征。【方法】分别参照GenBank中PEDV CV777株的N基因序列(AF353511)和编码人细胞核仁蛋白B23.1基因序列(BC050628.1),设计、合成扩增N基因和B23.1基因的引物,利用RT-PCR技术扩增了N基因和Vero E6细胞的B23.1基因的cDNA,分别克隆到真核表达载体pAcGFP1-C1和pDsRed2-N1,获得重组质粒pAcGFP1-C1/N和pDsRed2-N1/B23.1,共转染Vero E6细胞。【结果】Western blots分析表明这些融合蛋白在转染的Vero E6细胞中表达;共聚焦显微镜技术分析表明在共转染Vero E6细胞中猪流行性腹泻病毒N蛋白与Vero E6细胞核磷蛋白B23.1发生共定位。【结论】为进一步鉴定PEDV N蛋白中核仁定位信号和N蛋白核仁定位机制提供可靠依据。
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| 关 键 词： | 猪流行性腹泻病毒 核衣壳蛋白 核磷蛋白B23.1 共定位 |
| 收稿时间： | 2010/12/16 0:00:00 |
| 修稿时间： | 2011/2/24 0:00:00 |
Co-localization analysis between porcine epidemic diarrhea virus nucleocapsid protein and nucleolar phosphoprotein B23.1 |
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| Maojie Lv,Jianfei Chen,Hongyan Shi,Xiaojin Chen,Xiuping Fan,Shichuan Shen and Li Feng.Co-localization analysis between porcine epidemic diarrhea virus nucleocapsid protein and nucleolar phosphoprotein B23.1[J].Acta Microbiologica Sinica,2011,51(5):643-647. |
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| Authors: | Maojie Lv Jianfei Chen Hongyan Shi Xiaojin Chen Xiuping Fan Shichuan Shen and Li Feng |
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| Institution: | Department of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Department of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Department of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Department of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Department of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Department of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;Department of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China |
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| Abstract: | Objective]To elucidate the co-localization characteristic between porcine epidemic diarrhea virus(PEDV) N protein and B23.1 phosphoprotein.Methods]Two pairs of primers used to amplify N gene and B23.1 gene were designed and synthesized according to CV777 N gene sequence(AF353511) and human nucleolar phosphoprotein B23.1 gene sequence(BC050628.1),respectively.The PEDV N gene and B23.1 gene were amplified by RT-PCR from PEDV strain CV777 and Vero E6 cells,respectively;then cloned into eukaryotic expression ... |
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| Keywords: | porcine epidemic diarrhea virus nucleocapsid protein nucleolar phosphoprotein B23 1 co-localization |
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