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中国家猫克氏巴尔通体分离株生物学及分子特征分析
引用本文:栗冬梅,刘起勇,宋秀平,张建中,许传彬,杨晓冉.中国家猫克氏巴尔通体分离株生物学及分子特征分析[J].微生物学报,2009,49(4):429-437.
作者姓名:栗冬梅  刘起勇  宋秀平  张建中  许传彬  杨晓冉
作者单位:1. 中国疾病预防控制中心传染病预防控制所,北京,102206
2. 山东省烟台市经济技术开发区疾病预防控制中心,烟台,264006
3. 北京市公共卫生信息中心,北京,100050
摘    要:摘要:【目的】分析中国家猫中分离的巴尔通体菌株M9HN-SHQ生物学性状和分子特征。【方法】应用含5%羊血的胰酶大豆琼脂培养基在5% CO2培养箱中37℃培养6~7 d,革兰氏和吉姆尼茨染色镜下观察形态;应用VITEK ANI厌氧菌鉴定卡进行生化反应鉴定;气相色谱分析方法获取菌体脂肪酸成份组成(CFA);Etest药敏试条测定对10种抗生素的敏感性;分别应用随机扩增多态性DNA(RAPD)和脉冲场凝胶电泳(PFGE)技术对目标菌株和其他国际标准菌株等绘制DNA指纹图谱;对16S rRNA,gltA,gro

关 键 词:关键词:巴尔通体  生化鉴定  分子鉴定  猫抓病  
收稿时间:2008/10/13 0:00:00
修稿时间:2008/12/13 0:00:00

Biological and molecular characteristics of a cat-borne Bartonella clarridgeiae
Dongmei Li,Qiyong Liu,Xiuping Song,Jianzhong Zhang,Chuanbin Xu and Xiaoran Yang.Biological and molecular characteristics of a cat-borne Bartonella clarridgeiae[J].Acta Microbiologica Sinica,2009,49(4):429-437.
Authors:Dongmei Li  Qiyong Liu  Xiuping Song  Jianzhong Zhang  Chuanbin Xu and Xiaoran Yang
Institution:National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;Disease Control and Prevention Center of Economic and Technological Development District, Yantai 264006, China;Beijing Public Health Information Center, Beijing100050, China
Abstract:
Keywords:Keywords: Bartonella  biochemical identification  molecular identification  cat scratch disease
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