| 产气肠杆菌几丁质酶的分离纯化及性质研究 |
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| 引用本文： | 唐亚雄,赵建,丁诗华,刘世贵,杨志荣.产气肠杆菌几丁质酶的分离纯化及性质研究[J].微生物学报,2001,41(1):82-86. |
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| 作者姓名： | 唐亚雄 赵建 丁诗华 刘世贵 杨志荣 |
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| 作者单位： | 四川大学草原生物防治工程国家专业实验室,成都,610064 |
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| 摘 要： | 从自然罹病死亡的草原毛虫(Gynephorap ruoergnesis)体内分离到一株产气肠杆菌(Enterobacter aerogenes),它在几丁质的诱导下能产生较高活性的几丁质酶。发酵液经硫酸铵盐析、DEAE纤维素柱层析和Sephadex G-100柱层析分离出几丁质酶。用SDSPAGE测得该酶的分子量为425kD。水解几丁质的Km值为2.88mg/mL-1。酶反应的最适温度为55℃，最适pH值为60，金属离子对几丁质酶活性影响较大，其中Zn2+、Ba2+、Ca2+和Mn2+对酶有较强的激活作用，而Hg2+、Co2+和Mg2+则有较强的抑制作用。
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| 关 键 词： | 产气肠杆菌，几丁质酶，纯化，酶学性质 |
| 文章编号： | 0001-6209(2001)01-0082-05 |
| 修稿时间： | 1999年11月15 |
PURIFICATION AND PROPERTIES OF CHITINASE FROM ENTEROBACTER AEROGENES |
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| Tang Yaxiong\ Zhao Jian\ Ding Shihua\ Liu Shigui\ Yang Zhirong.PURIFICATION AND PROPERTIES OF CHITINASE FROM ENTEROBACTER AEROGENES[J].Acta Microbiologica Sinica,2001,41(1):82-86. |
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| Authors: | Tang Yaxiong\ Zhao Jian\ Ding Shihua\ Liu Shigui\ Yang Zhirong |
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| Institution: | National Laboratory of Grassland Biological Control, Sichuan University, Chengdu 610064. |
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| Abstract: | A bacterium producing chitinase was isolated from the dead body of Gymephorap ruoergensis. A chitinase was isolated from the culture of E. aerogenes and purified by means of ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-100 column gel filtration. The purified chitinase showed homogeneity on the native polyacrylamide gel electrophoresis. Its molecular weight was estimated to be about 42.5 kD by SDS-PAGE. The optimum pH and temperature for hydrolysis of chitin were 6.0 and 55 degrees C respectively. Michaelis constant was 2.88 mg/mL. Different metal ions showed different effects on the chitinase activity, The chitinase activity was enhanced by Zn2+, Ba2+, Ca2+, Mn2+ and was strongly inhibited by Hg2+, Co2+, Mg2+. |
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| Keywords: | Enterobacter aerogenes Chitinase Isolation and purification Properities |
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