| 乳链菌肽高产菌株的选育及其基因定位 |
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| 引用本文： | 还连栋,陶勇,何松,田宇清,薛禹谷.乳链菌肽高产菌株的选育及其基因定位[J].微生物学报,1995,35(5):364-367. |
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| 作者姓名： | 还连栋 陶勇 何松 田宇清 薛禹谷 |
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| 作者单位： | 中国科学院微生物研究所；中国科学院微生物研究所 北京 |
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| 基金项目： | 国家自然科学基金资助项目，中国科学院“八五”重点科研项目 |
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| 摘 要： | 以乳酸乳酸球菌7962为原始菌株,用紫外线、LiCl、(60)~Co及8-MOP+NUV等多种理化诱变剂对其进行诱变处理,获得一株乳链菌肽高产突变株AL2。其效价稳定在2300～2500Iu/ml。经DNA杂交证实,编码乳链菌肽的前体基因位于染色体上,其遗传性状是稳定的。毒理试验表明AL2及其产物属于实际无毒类物质。
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| 关 键 词： | 乳酸乳酸球菌 乳链菌肽 诱变育种 基因定位 |
THE SCREENING OF HIGH-YIELD NISIN PRODUCING STRAINS AND LOCALIZATION OF PRECURSOR NISIN GENE |
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| Huan Liandong Tao Yong He Song Tian Yuqing Xue Yugu.THE SCREENING OF HIGH-YIELD NISIN PRODUCING STRAINS AND LOCALIZATION OF PRECURSOR NISIN GENE[J].Acta Microbiologica Sinica,1995,35(5):364-367. |
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| Authors: | Huan Liandong Tao Yong He Song Tian Yuqing Xue Yugu |
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| Abstract: | A mutant strain AL2 with high yield of nisin was obtained from Lacto-coccus lactis 7962 by treatment with physical and chemical factors. The nisin activities of fermentation medium were 2300-2500 IU/ml. LD50 test results indicated that AL2 strain and its product (nisin) are nontoxic. Hybridization experiments suggested that the presence of precursor nisin gene is on the chromosome. |
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| Keywords: | Lactococcus lactis Nisin Mutation breeding Gene localization |
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