|
|||||
|
|
| 一株产冠菌素新菌种的分离与鉴定 | |
| 引用本文： | 王园秀,吴晓玉,丰娟,曾晓春,饶志强.一株产冠菌素新菌种的分离与鉴定[J].微生物学报,2010,50(1):23-28. |
| 作者姓名： | 王园秀 吴晓玉 丰娟 曾晓春 饶志强 |
| 作者单位： | 1. 江西农业大学生物科学与工程学院,南昌330045;南昌市发酵应用技术重点实验室,南昌330045 2. 江西农业大学生物科学与工程学院,南昌330045;宜春学院生命科学与资源环境学院,南昌330045 3. 江西农业大学农学院,南昌,330045 |
| 基金项目： | 博士启动基金，江西省自然科学基金（2008GJN0016）及国家自然科学基金（30760113） |
| 摘 要： | 【目的】从不同样本中分离筛选性能稳定的产冠菌素菌株。【方法】根据冠菌素引起植物叶片产生弥散性黄萎病、块茎膨大的特性,采集各种植物病叶、病枝及感病植物的土壤,采用穿刺法与系列稀释法分离筛选菌株;液相色谱测定菌株产生的冠菌素;在电子和光学显微镜下观察菌体形态;根据生理生化试验、(G+C)mol%含量、16S rDNA序列分析等对菌株进行鉴定;对分离提纯的发酵产物进行紫外、质谱和红外分析。【结果】菌株BBC933为革兰氏阴性菌,端生鞭毛,短杆状,无芽孢。在41℃下不生长,细胞内有聚β-羟基丁酸盐颗粒积累,没有精氨酸双水解酶和氧化酶,不能水解淀粉、明胶,不进行硝酸还原及反硝化作用,过氧化氢酶反应呈阳性。菌株的(G+C)mol%含量为67.2%,根据该菌株16S rDNA序列的同源性分析,构建系统发育树。【结论】菌株BCC933鉴定为洋葱伯克霍尔德氏菌(Burkholder cepacia),具有产冠菌素性能。国内外未曾见报道洋葱伯克霍尔德氏菌产冠菌素。 |
| 关 键 词： | 关键词：冠菌素 伯克霍尔德菌 鉴定 16S rDNA |
| 收稿时间： | 2009/6/22 0:00:00 |
| 修稿时间： | 2009/9/23 0:00:00 |
Isolation and identification of a coronatine producing strain |
|
| Yuanxiu Wang,Xiaoyu Wu,Juan Feng,Xiaochun Zeng and Zhiqiang Rao.Isolation and identification of a coronatine producing strain[J].Acta Microbiologica Sinica,2010,50(1):23-28. | |
| Authors: | Yuanxiu Wang Xiaoyu Wu Juan Feng Xiaochun Zeng and Zhiqiang Rao |
| Institution: | College of Bioscience and Bioengineering of Jiangxi Agricultural University, Nanchang 330045, China;College of Bioscience and Bioengineering of Jiangxi Agricultural University, Nanchang 330045, China;College of Bioscience and Bioengineering of Jiangxi Agricultural University, Nanchang 330045, China;College of Agronomy of Jiangxi Agricultural University, Nanchang 330045, China;College of Bioscience and Bioengineering of Jiangxi Agricultural University, Nanchang 330045, China |
| Abstract: | Objective] We screened and isolated coronatine-producing stains from various samples. Methods] The strains were isolated and selected from samples by the methods of streak plate and serial dilution. The samples were sick leaves/branches and soil in which plants got sick according to the symptoms of leaf blight disease and tuber enlargement. Coronatine production was determined by high performance liquid chromatography (HPLC). The strain was characterized by the physiological and biochemical analysis,the d... |
| Keywords: | Keywords: Coronatine Burkholderia identification 16S rDNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《微生物学报》浏览原始摘要信息 | |
| 点击此处可从《微生物学报》下载免费的PDF全文 | |