基于PCR-DGGE技术的红树林区微生物群落结构

【目的】为了解红树林沉积物中细菌的群落结构特征。【方法】应用PCR-DGGE技术对福建浮宫红树林的16个采样站位样品细菌的群落结构进行了研究。根据DGGE指纹图谱,对它们的遗传多样性进行了分析。【结果】各站位样品细菌多样性指数(H)、丰度(S)和均匀度(EH)均有所不同,这些差异与它们所处站位的不同有关,红树林区细菌多样性高于非红树林区细菌多样性。对不同站位细菌群落相似性分析,它们的相似性系数也存在一定的规律,同一断面的细菌群落结构相近性较高。对DGGE的优势条带序列分析,同源性最高的微生物分别属于变形菌门(Proteobacteria)、酸菌门(Acidobacteria)和绿菌门(Chlorobi),它们均为未培养微生物,分别来自于河口海岸沉积物。【结论】应用PCR-DGGE技术更能客观地反映红树林沉积物中真实的细菌群落结构信息。另外,研究也表明红树林区微生物多样性丰富,在红树林区研究开发未知微生物资源具有巨大的潜力。

Analysis of microbial community structure in mangrove sediments by PCR-DGGE technique

Abstract: Objective] To understand the microbial community in mangrove sediments. Methods] Sixteen stations were established in the Fugong mangrove area of Jiulong River Estuary, Fujian, China. Microbial community structure in this area was evaluated by PCR-DGGE method. The genetic diversity of microorganisms was analyzed based on the DGGE fingerprint. Results] There were significant differences among the Shannon-wiener index, Richness and evenness. The possible reason might due to distinct location of samples from different stations. Bacterial diversity was higher in the mangrove areas than that in non-mangrove areas. Similarity analysis of bacterial communities in different station showed certain trends in similarity coefficients, and bacterial community structure similarity of the same transect was much higher than others. According to the results of sequence analysis of DGGE dominant bands, all of them were phylogenetically close to Proteobacteria, Acidobacteria and Chlorobia which belonged to uncultured microbes from coastal sediments in the estuary. Conclusion] There are abundant microbial diversity and a large number of unknown microbial resources in mangrove sediments which need further research.