固定化啤酒酵母细胞催化有机硅酮不对称还原

研究了固定化啤酒酵母细胞催化三甲基硅乙酮不对称还原反应，系统探讨了振荡速度、底物浓度、固定化细胞浓度、pH值和反应温度对反应速度、产率和产物光学纯度的影响。结果表明，上述因素对固定化啤酒酵母细胞催化三甲基硅乙酮不对称还原反应均有较显著的影响。振荡速度以150r/min为宜，底物浓度和固定化细胞浓度分别为14mmol/L和0.15g/mL较佳，适宜的pH值为7.3，最佳反应温度为25℃～30℃。在该优化反应条件下，反应最大产率和产物的光学纯度分别高达84.9%和90.2%ee。

Asymmetric Microbial Reduction of Organosilyl Ketone with Immobilized Saccharomyces cerevisiae Cells

Asymmetric microbial reduction of acetyltrimethylsilane with immobilized Saccharomyces cerevisiae cells was studied. The effects of shake speeds, substrate concentration, immobilized cell concentration, pH and reaction temperature on the initial reaction rate, maximum yield and optical purity of the product were systematically studied. All above mentioned parameters have important effects on the raction. The optimum shake speed, substrate concentration, immobilized cell concentration, pH and reaction temperature were found to be 150 r/min, 14 mmol/L, 0.15 g/mL, 7.3 and 25 degrees C-30 degrees C respectively for the reaction, under which the maximum yield and optical purity of the product were as high as 84.9% and 90.2% ee.