| 链霉菌Z94-2碱性脂肪酶产生条件及酶学性质 |
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| 引用本文： | 周晓云,黄建宁,欧志敏,王慧中,王荣伟.链霉菌Z94-2碱性脂肪酶产生条件及酶学性质[J].微生物学报,2000,40(1):75-79. |
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| 作者姓名： | 周晓云 黄建宁 欧志敏 王慧中 王荣伟 |
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| 作者单位： | [1]浙江工业大学生物工程系,杭州 [2]浙江省农业科学院微生物研究所,杭州 |
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| 基金项目： | 浙江省科委重点项目和浙江省自然科学基金项目(397040) |
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| 摘 要： | 在１５２株脂肪酶产生菌中，链霉菌Ｚ９４－２产脂肪酶活力为５９６ｕ／ｍＬ，其最适培养基（ｇ／Ｌ）为：糊精１０、黄豆饼粉３０、尿素１０、Ｋ２ＨＰＯ４０．５、ＭｇＳＯ４０．５、ＮａＣｌ１和ＡＥＯ９０．５，产酶的最适条件为：初始ｐＨ９．５～１０．０，在２６℃培养４８ｈ。用ＰＶＡ橄榄油乳化系统测定该酶的最适ｐＨ９．８，最适温度３７℃，在ｐＨ８．６～１０．２于５℃存放２４ｈ，酶活力不变。０．１４ｍｏｌ／Ｌ的氯
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| 关 键 词： | 碱性脂肪酶 链霉菌 产酶条件 酶学性质 |
| 文章编号： | 0001-6209(2000)01-0075-79 |
CONDITIONS OF ENZYME PRODUCTION AND PROPERTIES OF ALKALINE LIPASE BY STREPTOMYCES Z94-2 |
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| Zhou Xiaoyun,Huang Jianning,Ou Zhimin,Wang Huizhong,Wang Rongwei.CONDITIONS OF ENZYME PRODUCTION AND PROPERTIES OF ALKALINE LIPASE BY STREPTOMYCES Z94-2[J].Acta Microbiologica Sinica,2000,40(1):75-79. |
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| Authors: | Zhou Xiaoyun Huang Jianning Ou Zhimin Wang Huizhong Wang Rongwei |
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| Institution: | Zhou Xiaoyun
;(Department of Biology Engineering, Zhejiang University of Technology, HangZhou 310014);Huang Jianning
;(Institute of Microbiology, Zhejiang Academy of Agriculture Sciences, HangZhou 310021);Ou Zhimin
;(Department of Biology Engineering, Zhejiang University of Technology, HangZhou 310014);Wang Huizhong
;(Department of BioloKy, Hang Zhou Normal College, Hangzhou 310012);Wang Rongwei
;(Department of Biology Engineering, Zhejiang University of Technology, HangZhou 310014) |
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| Abstract: | An alkaline lipase producing strain streptomyces Z94-2 was selected from 152 lipase producer, The optimal medium composition for the producing lipase is (g/L): soybean meal 30, urea 10, dextrin 10, KH2PO4 0.5, NaCl 1, MgSO4.7H2O 0.5, AEO9 (nonionic surfactant) 1. The optimal conditions for lipase producing are initial pH 9.5-10.0, shaken at 26 degrees C, for 48 h, a maximal lipase activity of reached 596 (u/mL). The optimal activity at pH 9.8 and at 37 degrees C, respectively. The lipase was stable at pH 8.6-10.2 and below 60 degrees C, and the lipase was highly activated by 0.14 mol/L CaCl2. |
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| Keywords: | Alkaline lipase Streptomyces Conditions of enzyme production Properties of enzyme |
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