| 产β-葡聚糖酶菌种T199的选育及发酵条件 |
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| 引用本文： | 石家骥,崔福绵.产β-葡聚糖酶菌种T199的选育及发酵条件[J].微生物学报,2001,41(6). |
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| 作者姓名： | 石家骥 崔福绵 |
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| 作者单位： | 中国科学院微生物研究所, |
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| 摘 要： | A mutant strain T199 producing about 8 times as much β-glucanase as its parent strain trichoderma kningii T3 was obtained by treatment with ultraviolet light and N-methyl-N-nitro-N-nitrosoguanidine.Fermentation was conducted in 250mL flask,each containing 30mL of medium consisted of 5% corn cob powder,3% wheat bran and 1.4% nitrogen source No.10 ((NH 4) 2SO 4 10%,Peptone 20%,Yeast extract 15%).The optima culture conditions were as below:initial pH5.0,30℃,shaking speed 280r/min,and cultivation time 5d.En…
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| 关 键 词： | 康宁木霉 β-葡聚糖酶 纤维素酶 昆布多糖酶 地衣多糖酶 |
SELECTION OF β-GLUCANASE-PRODUCING TRICHODERMA KoNINGII T199 AND ITS FERMENTATION CONDITIONS |
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