| 集胞藻PCC 6803中丝氨酸/苏氨酸激酶SpkC对高温胁迫的响应 |
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| 引用本文： | 赵佳琳,陈军,崔玉琳,于淑贤,陈高,秦松.集胞藻PCC 6803中丝氨酸/苏氨酸激酶SpkC对高温胁迫的响应[J].微生物学报,2018,58(10):1732-1742. |
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| 作者姓名： | 赵佳琳 陈军 崔玉琳 于淑贤 陈高 秦松 |
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| 作者单位： | 中国科学院烟台海岸带研究所;中国科学院大学;山东省农业科学院生物技术研究中心;山东省作物遗传改良与生态生理重点实验室 |
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| 基金项目： | 国家自然科学基金（41406192，41176144，41376139）；国家重点研发计划（2016YFF0202304）；烟台市科技计划（2016JHZB007） |
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| 摘 要： | 丝氨酸/苏氨酸激酶是蓝藻感知和转导外界刺激的重要元件,但至今蓝藻中很多丝氨酸/苏氨酸激酶的功能尚属未知。【目的】研究集胞藻PCC6803中的丝氨酸/苏氨酸激酶Spk C是否参与对高温胁迫的响应。【方法】本研究采用同源重组的方法构建spC基因完全敲除突变株,检测突变株与野生株在高温胁迫下的生长状况、色素组成,并对高温胁迫下叶绿素荧光参数差异进行分析,比较光合系统Ⅱ活性差异。此外,通过测定生长速率来判断高温胁迫后藻株的恢复情况。【结果】经过42℃高温胁迫后,与野生株相比,突变株ΔspkC生长减缓,光合色素(叶绿素、类胡萝卜素和藻胆色素)的含量降低;45℃高温胁迫下突变株ΔspkC的光合系统Ⅱ活性下降幅度更大;经过5 d 42℃高温处理后,突变株生长几乎停滞,存活率较野生株明显降低。【结论】集胞藻PCC 6803中spkC基因的缺失导致突变株对高温胁迫响应出现缺陷,提示丝氨酸/苏氨酸激酶SpkC参与响应高温胁迫。
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| 关 键 词： | 集胞藻PCC 6803 丝氨酸/苏氨酸激酶SpkC 高温胁迫 |
| 收稿时间： | 2017/11/7 0:00:00 |
| 修稿时间： | 2018/1/24 0:00:00 |
Response of serine/threonine protein kinase SpkC to high temperature stress in Synechocystis sp. PCC 6803 |
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| Jialin Zhao,Jun Chen,Yulin Cui,Shuxian Yu,Gao Chen and Song Qin.Response of serine/threonine protein kinase SpkC to high temperature stress in Synechocystis sp. PCC 6803[J].Acta Microbiologica Sinica,2018,58(10):1732-1742. |
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| Authors: | Jialin Zhao Jun Chen Yulin Cui Shuxian Yu Gao Chen and Song Qin |
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| Institution: | Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, Shandong Province, China;University of Chinese Academy of Sciences, Beijing 100049, China,Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, Shandong Province, China,Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, Shandong Province, China,Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, Shandong Province, China,Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China;Shandong Provincial Key Laboratory of Genetic Improvement, Ecology and Physiology of Crops, Jinan 250100, Shandong Province, China and Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, Shandong Province, China |
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| Abstract: | |
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| Keywords: | Synechocystis sp PCC 6803 serine/threonine protein kinase SpkC high temperature stress |
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