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恶臭假单胞菌NA-1菌株烟酸羟基化酶活性的诱导和转化条件的研究
引用本文:陆伟宏,王鑫,徐莉,戴亦军,袁生. 恶臭假单胞菌NA-1菌株烟酸羟基化酶活性的诱导和转化条件的研究[J]. 微生物学报, 2005, 45(4): 551-555
作者姓名:陆伟宏  王鑫  徐莉  戴亦军  袁生
作者单位:南京师范大学生命科学学院,江苏省资源生物技术重点实验室,南京师范大学微生物工程重点实验室,南京,210097
基金项目:国家“十五”科技攻关项目(2001BA308A05-02)~~
摘    要:恶臭假单胞菌NA-1菌株的培养和产酶特性与已报道的产酶菌株粘质沙雷氏菌(Serratiamarcescens)IFO12648和荧光假单胞菌(Psudomonasfluorescens)TN5有所不同,主要反映在最适碳源及浓度、最适诱导剂浓度和最适培养温度等方面。最适的转化条件是温度为30℃,pH为7.0,烟酸的浓度为3%。采用初步优化后的条件和流加底物的方式进行4L上罐生产,恶臭假单胞菌NA-1菌株的6-羟基烟酸产率可达到108.39gL。

关 键 词:烟酸  6-羟基烟酸  生物转化  恶臭假单孢菌
文章编号:0001-6209(2005)04-0551-05
修稿时间:2004-11-15

Induction of nicotinic acid hydroxylase activity of Pseudomonas putida NA-1 and optimization of transformation conditions
LU Wei-hong,WANG Xin,XU Li,DAI Yi-jun,Yuan Sheng. Induction of nicotinic acid hydroxylase activity of Pseudomonas putida NA-1 and optimization of transformation conditions[J]. Acta microbiologica Sinica, 2005, 45(4): 551-555
Authors:LU Wei-hong  WANG Xin  XU Li  DAI Yi-jun  Yuan Sheng
Affiliation:Jiangsu Provincial Key Laboratory for Bioresource Technology, College of Life Science, Nanjing Normal University, Nanjing 210097, China. luweihong168@sohu.com
Abstract:Some features of Pseudomonas putida NA-1 strain in cultivation and enzyme production were different from S. marcescens IFO 12648 and P. fluorescens TN5 stains which could transform nicotinic acid to 6-hydroxynicotinic acid reported by other scientists, such as optimal carbon and its optimal concentration, optimal inducer concentration, and optimal cultivation temperature. The ideal transformation condition was nicotinic acid 3%, temperature 35 degrees C and pH 7.0. Under an appropriate condition, in a 4 liter fermenter, production yield of 6-hydroxynicotinic acid by Pseudomonas putida NA-1 was 108.39 g/L.
Keywords:Nicotinic acid   6-hydroxynicotinic acid   Biotransformation   Pseudomonas putida
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