| 恶臭假单胞菌NA-1菌株烟酸羟基化酶活性的诱导和转化条件的研究 |
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| 引用本文： | 陆伟宏,王鑫,徐莉,戴亦军,袁生. 恶臭假单胞菌NA-1菌株烟酸羟基化酶活性的诱导和转化条件的研究[J]. 微生物学报, 2005, 45(4): 551-555 |
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| 作者姓名： | 陆伟宏 王鑫 徐莉 戴亦军 袁生 |
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| 作者单位： | 南京师范大学生命科学学院,江苏省资源生物技术重点实验室,南京师范大学微生物工程重点实验室,南京,210097 |
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| 基金项目： | 国家“十五”科技攻关项目(2001BA308A05-02)~~ |
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| 摘 要： | 恶臭假单胞菌NA-1菌株的培养和产酶特性与已报道的产酶菌株粘质沙雷氏菌(Serratiamarcescens)IFO12648和荧光假单胞菌(Psudomonasfluorescens)TN5有所不同,主要反映在最适碳源及浓度、最适诱导剂浓度和最适培养温度等方面。最适的转化条件是温度为30℃,pH为7.0,烟酸的浓度为3%。采用初步优化后的条件和流加底物的方式进行4L上罐生产,恶臭假单胞菌NA-1菌株的6-羟基烟酸产率可达到108.39gL。
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| 关 键 词： | 烟酸 6-羟基烟酸 生物转化 恶臭假单孢菌 |
| 文章编号： | 0001-6209(2005)04-0551-05 |
| 修稿时间： | 2004-11-15 |
Induction of nicotinic acid hydroxylase activity of Pseudomonas putida NA-1 and optimization of transformation conditions |
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| LU Wei-hong,WANG Xin,XU Li,DAI Yi-jun,Yuan Sheng. Induction of nicotinic acid hydroxylase activity of Pseudomonas putida NA-1 and optimization of transformation conditions[J]. Acta microbiologica Sinica, 2005, 45(4): 551-555 |
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| Authors: | LU Wei-hong WANG Xin XU Li DAI Yi-jun Yuan Sheng |
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| Affiliation: | Jiangsu Provincial Key Laboratory for Bioresource Technology, College of Life Science, Nanjing Normal University, Nanjing 210097, China. luweihong168@sohu.com |
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| Abstract: | Some features of Pseudomonas putida NA-1 strain in cultivation and enzyme production were different from S. marcescens IFO 12648 and P. fluorescens TN5 stains which could transform nicotinic acid to 6-hydroxynicotinic acid reported by other scientists, such as optimal carbon and its optimal concentration, optimal inducer concentration, and optimal cultivation temperature. The ideal transformation condition was nicotinic acid 3%, temperature 35 degrees C and pH 7.0. Under an appropriate condition, in a 4 liter fermenter, production yield of 6-hydroxynicotinic acid by Pseudomonas putida NA-1 was 108.39 g/L. |
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| Keywords: | Nicotinic acid 6-hydroxynicotinic acid Biotransformation Pseudomonas putida |
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