江苏无锡健康与肠病人群肠道脱硫弧菌数量及肠道菌群多样性

目的]研究息肉、溃疡性结肠炎、直肠结肠癌和健康人群肠道中脱硫弧菌数量的差异,及不同人群肠道菌群的多样性,分析脱硫弧菌数量及肠道菌群多样性与肠道疾病之间的潜在关系.方法]采用实时荧光定量PCR(RT-PCR)的方法,对58名受试者肠道脱硫弧菌的数量进行定量分析.采用PCR-DGGE技术,对不同人群的肠道脱硫弧菌和肠道菌群结构进行分析,结合16S rRNA V3区测序分析不同人群肠道菌群多样性的差异.结果]RT-PCR分析显示,所有受试者均为脱硫弧菌阳性,其中息肉(2.9×106cfu/mL)和溃疡性结肠炎人群(1.2×106 cfu/mL)肠道中脱硫弧菌的数量明显高于健康人群(7.0×105 cfu/mL),直肠结肠癌人群(6.8×105 cfu/mL)肠道中脱硫弧菌的数量与健康人群无明显差异.DGGE图谱聚类分析结果表明,肠道疾病人群肠道中脱硫弧菌的菌群相似度较高,而与健康人群之间的差异较大.16S rRNA V3区基因测序显示肠道疾病人群与健康人群在肠道菌群多样性和优势菌群方面均有明显差异.结论]通过RT-PCR与DGGE相结合的方法,说明肠道脱硫弧菌数量的增多是息肉和溃疡性结肠炎疾病的一个重要特征,且其菌群组成在肠道疾病人群与健康人群之间存在明显差异.与健康人群相比,肠道疾病人群的肠道微生物多样性升高,优势菌群发生偏移,菌群失衡.

Quantity of Desulfovibrios and analysis of intestinal microbiota diversity in health and intestinal disease people in Wuxi,Jiangsu province

Objective]This paper provides an overview of Desulfovibrio(DSV) incidence and its effect on bacterial diversity in human gastrointestinal tract of four groups: ulcerative colitis(UC),colorectal cancer(CRC),polypus(PP) and the healthy control(H).Methods] Real time fluorescence quantitative PCR(RT-PCR) assays were used to enumerate DSV in gastrointestinal tract of 58 subjects.Diversity of gut microbiota was analyzed by PCR-Denaturing Gradient Gel Electrophoresis(PCR-DGGE) and 16S rRNA V3 sequencing.Results] RT-PCR detected DSV in all samples.Significantly increased numbers of DSV were observed for UC and PP groups compared with CRC and H groups.No significant difference was observed for CRC and H groups with gene copy numbers of DSV.Alterations of DSV and gut microbiota were observed in disease groups.Conclusion] We found that quantity and diversity of DSV are significantly increased in UC and PP compared to controls.The increased numbers of DSV in disease groups suggests a possible harmful role.