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鸡贫血病毒哈尔滨分离株全基因克隆和序列分析
引用本文:何成强,丁乃峥,李景鹏,李云龙.鸡贫血病毒哈尔滨分离株全基因克隆和序列分析[J].微生物学报,2002,42(4):436-441.
作者姓名:何成强  丁乃峥  李景鹏  李云龙
作者单位:1. 山东师范大学生物系,济南,250014;东北农业大学生命科学学院,哈尔滨,150030
2. 东北农业大学生命科学学院,哈尔滨,150030
3. 山东师范大学生物系,济南,250014
基金项目:黑龙江科委攻关项目 :编号 97SG74SG0 4
摘    要:通过PCR方法克隆了从哈尔滨分离的一株鸡贫血病毒(CAV)的全基因,并对其进行了测序,该病毒基因组为环状,全长2298bp,含有三个互相重叠的开放读码框和一个调控区。将克隆的基因与GenBank收录的CAV基因比较,同源性至少为97%,未发现与本次克隆的CAV基因完全一致的分离株。与德国分离株Cuxla、26p4和马来西亚分离株分别有42、42和72个核苷酸不同,同源性分别为98.2%、98.2%和96.9%。与德国分离株Cux1b相比,除在调控区内少一个类似增强子的重复序列外,尚有39处核苷酸不同。它与分离于欧洲的几株CAV的亲源性要比来自亚洲的马来西亚株近。对CAV哈尔滨分离株、26p4、Cux1b、Cux1a和马来西亚株的VP1、VP2和VP3蛋白比较,VP2的保守性最高。

关 键 词:PCR,  鸡贫血病毒,  基因组
文章编号:0001-6209(2002)04-0436-06

Gene Cloning and Sequencing of Chicken Anemia Virus(CAV) Isolated From Harbin
Chengqing He,Naizheng Ding,Jingpeng Li,Yunlong Li.Gene Cloning and Sequencing of Chicken Anemia Virus(CAV) Isolated From Harbin[J].Acta Microbiologica Sinica,2002,42(4):436-441.
Authors:Chengqing He  Naizheng Ding  Jingpeng Li  Yunlong Li
Institution:Biologic Department of Shandong Normal University, Jinan 250014, China.
Abstract:A Chicken anemia virus has been isolated from a chicken flock in Harbin of China.The genome of the ivrus was cloned through polymerase chain reaction(PCR) and sequence of the genome was analyzed.The cycle genome is made of 2298 base pairs including three overlapping open reading frames(vp1,vp2,vp3)and a regulative region.Comparing sequence of the genome through BLAST in GenBank,this sequence exhibits 96.9% identity with other genome of CA Vs and least.Multiple alignment of this genome of this virus, 26p4,strain isolated in Germany,strain isolated in Malaysia and Cux 1 found that this sequence exhibits 98.2%(42/2298),98.2%(42/2298),96.9%(72/2298)and 97.5%(60/2319)identify with them,respectively.A new CAV strain was isolated and it has better identify with CAV isolated in Europe countries than is Asia country Malaysia.Multiple alignment of VP1,VP2,VP3 of 26p4,strain isolated in Germany,strain isolated in Malaysia,Cux 1 and strain isolated in Harbin of China found the VP2 the most conservative.
Keywords:Polymerase chain reaction  Chicken anemia virus  Genome  
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