从传代细胞株中去除支原体污染的研究

用抗菌药物从传代细胞株中去除支原体污染的试验结果表明，卡那霉素和庆大霉素对支原体均无明显的杀灭作用。用lOμg／ml的Tiamutin处理支原体的效果较好。采用单克隆细胞稀释、选择法与Tiamutin(10μg／ml)处理相结合，经电镜观察可去除细胞中支原体的污染。检查支原体的方法是否特异、敏感、快速是对试验结果正确判断的一个重要的关键。为了防止支原体的污染，除了加强对原材料(包括小牛血清、培养液等)的检查以及把住严格的无菌操作条件外，必要时可在培养基中加入10μg／ml的Tiamutin。

Studies on elimination of mycoplasmas from cell line cultures

Mycoplasmas were effectively eliminated from contaminated LTK and CHO cell lines by means of the antibiotic Tiamutin (10 micrograms/ml), while Kanamycin and Gentamycin were less potential. An elimination procedure which involved the consecutive treatment of the cell cloning over a periods of 21-28 days was adopted. This procedure was effective when applied to cell lines contaminated with unidentified and partially non-cultivable strains of mycoplasmas which could be detected by electronic microscope.