| 芽孢杆菌M50产生β—甘露聚糖酶的条件研究 |
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| 引用本文： | 陈一平,龙健儿.芽孢杆菌M50产生β—甘露聚糖酶的条件研究[J].微生物学报,2000,40(1):62-68. |
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| 作者姓名： | 陈一平 龙健儿 |
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| 作者单位： | 中国科学院成都生物研究所,成都 |
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| 基金项目： | 四川省“九．五”攻关项目(1010801)及中国科学院地奥科学基金(970370) |
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| 摘 要： | 从土壤中分离到９株产生β－甘露聚糖酶的芽孢杆菌（Ｂａｃｉｌｌｕｓ ｓｐ．）。Ｂａｃｉｌｌｕｓ ｓｐ．Ｍ５０２５０ｍＬ三角瓶摇瓶培养试验，以４％的魔芋粉为碳源，１．０％（ＮＨ４）２ＳＯ４为氮源，０．３５％Ｎａ２ＣＯ３，３０～３４℃培养６０ｈ产酶达到高峰。酶活力为１８０～２２０ｕ／ｍＬ。１００Ｌ罐发酵，在３０～３２℃，１：０．７５ｖｖｍ通气量，２００ｒ／ｍｉｎ条件下，发酵液酶活力高达３３０ｕ／ｍＬ。
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| 关 键 词： | β－甘露聚糖酶 芽孢杆菌 脱胶 产酶菌 产酶条件 |
| 文章编号： | 0001-6209(2000)01-0062-68 |
Study on the production of beta-mannanase by Bacillus M50] |
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| Chen Yiping
,Long Jianer
,Liao Lianhua
,Zhang Yunqiang
,Yang Jia.Study on the production of beta-mannanase by Bacillus M50][J].Acta Microbiologica Sinica,2000,40(1):62-68. |
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| Authors: | Chen Yiping Long Jianer Liao Lianhua Zhang Yunqiang Yang Jia |
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| Institution: | Chen Yiping
,Long Jianer
,Liao Lianhua
,Zhang Yunqiang
,Yang Jia
;(Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041) |
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| Abstract: | Bacillus sp. M50 produced beta-mannanase efficiently in the medium composed of 4% konjac powder, 1.0% (NH4)2SO4 and 0.35% Na2CO3. Fermentation of beta-mannanase by Bacillus sp. M50 has been carried out in 100 L tank. The temperature, ventitation quantity and agitation speed were 30-32 degrees C, 1:0.75 vvm and 220 r/min respectively. the fermentation cycle was 28 h and the highest beta-mannanase activity was 330 u/mL. The optimal temperature and pH for beta-mannanase reaction were 50 degrees C and 6.0 respectively. It was stable at pH 4.0-7.0 and below 50 degrees C. The activity of the enzyme was strongly inhibited by Fe3+, Al3+, EDTA, Hg2+ and slightly stimulated by Ba2+, Mn2+. Treatment of ramie-fibers with crude enzyme preparations from Bacillus sp. M50 showed a good degradation for hemicellulose of ramie-fibers residues. |
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| Keywords: | Mannanase Bacillus sp Degumming |
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