含有禽网状内皮组织增生病病毒基因组片段的天然重组禽痘病毒的研究

将国内5个不同生产厂家来源的禽痘弱毒疫苗株和1株禽痘野毒株在鸡胚成纤维细胞(CEF)上连续传5代，用禽网状内皮组织增生病病毒(Reticuloendotheliosis virus，REV)特异性的单克隆抗体进行间接免疫荧光试验(Immunonuoreseellee assay，IFA)，均检测不到传染性REV。但以6株禽痘病毒(FPV)感染的第2代和第5代细胞基因组提取物为模板，通过PCR均能扩增出REV的长末端重复序列(LTR)和囊膜蛋白(env)基因片段。用特异性核酸探针作分子斑点杂交(Dot blot)，结果显示所扩增的PCR条带为特异的REV—LTR和REV—env)基因片段。实验结果表明，国内的一些痘病毒疫苗和野毒株基因组中，已稳定地整合进了REV的基因组成分。

Detection of Reticuloendotheliosis Virus Genomic Sequence in The Genome of Field and Vaccine Strains of Fowl Poxvirus

Integration of reticuloendotheliosis virus (REV) into the genome of fowl poxvirus (FPV) has been reported recently. In order to determine whether this event has occurred in China, the presence of long terminal repeat (LTR) and envelop (env) gene fragments of REV provirus were detected from genomes of five FPV vaccines and a field isolate. When these six FPV strains were inoculated in the Chicken Embryo Fibroblast (CEF) cells and passed for 5 times respectively, the infectious REV could not be detected in immunofluorescence assay (IFA) with REV specific monoclonal antibody (MAb) 11B118. REV-LTR and REV-env gene fragments were amplified from the DNA extracts of the passage 2 and the passage 5 of FPV-infected CEF cells by PCR for all the 6 strains. Dot blot test proved the presence of LTR and env gene in the FPV-infected cell extracts and the PCR product for REV-LTR and REV-env. The results indicated that stable integrations of REV gene fragments in the genome of FPV vaccine and field strains were very common in China.