| 猪瘟病毒的形态结构与形态发生 |
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| 引用本文： | 王镇,闵光伟,李明义,藤俊琳,丁明孝.猪瘟病毒的形态结构与形态发生[J].微生物学报,2000,40(3):237-242. |
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| 作者姓名： | 王镇 闵光伟 李明义 藤俊琳 丁明孝 |
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| 作者单位： | 北京大学生命科学学院,北京,100871 |
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| 基金项目： | 国家攀登计划(85-44-02-05)和国家重点基础研究发展规划项目资助 |
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| 摘 要： | 建立了猪瘟病毒(CSFV)弱毒疫苗Thiverval株(T株)与中
国兔化弱毒疫苗C株在MPK细胞中的感染模式。使用MPK细胞增殖CSFV，其病毒滴度明显提高，从而为应用电镜超薄切片技术研究猪瘟病毒的形态结构与形态发生提供了可能性。猪瘟病毒呈圆形颗粒，直径约为70nm。其内部是电子致密的核心，直径约为40nm，有时呈六角形；外有包膜包裹。在CSFV感染的MPK细胞质中，可观察到处于不同发育阶段的子代病毒粒子。此外，猪瘟病毒的感染能够引起某些宿主细胞超微结构上的变化。
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| 关 键 词： | 猪瘟病毒(CSFV)， 超微结构， 形态发生， 电子显微镜技术 |
| 文章编号： | 0001-6209(2000)03-0237-42 |
STUDY ON THE MORPHOLOGICAL PROCESSING OF CLASSICAL SWINE FEVER VIRUS IN CULTURED CELLS |
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| Wang Zhen,Min Guangwei,Li Mingyi,Teng Junlin,Ding Mingxiao.STUDY ON THE MORPHOLOGICAL PROCESSING OF CLASSICAL SWINE FEVER VIRUS IN CULTURED CELLS[J].Acta Microbiologica Sinica,2000,40(3):237-242. |
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| Authors: | Wang Zhen Min Guangwei Li Mingyi Teng Junlin Ding Mingxiao |
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| Institution: | Wang Zhen
,Min Guangwei
,Li Mingyi
,Teng Junlin
,Ding Mingxiao
;(College of life sciences, Peking University, Beijing 100871) |
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| Abstract: | An infected mode between the Thiverval strain and Chinese strain of CSFV and MPK cells are established. The morphological structure and processing of different strains(Tstrain, C strain and F strain) of CSFV were studied by ultra-thin section electron microscopy. The virions of CSFV are roughly round and approximately 70nm in diameter with a 40nm core, and are wraped by membrance. The distinctive pathway of the maturation and release of CSFV are observed. Virons on different deverloping state and the pathologic changes of host cells have also been reported in this paper. |
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| Keywords: | Classical Swine Fever Virus(CSFV) Ultra-structure Morphological processing Electron microscopy |
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