角蛋白酶生产菌株的分离筛选与鉴定

【目的】分离筛选具有高效脱毛能力的野生角蛋白酶生产菌株,开发无硫制革生物脱毛剂。【方法】以贮备原料皮的特定环境中的污水样品为菌株源、在含诱导物脱脂羊毛粉的培养基中的富集、筛选与评估其发酵液脱毛能力的多相筛选方法分离选育高产角蛋白酶野生菌株。通过形态学、生理生化特征,Biolog全自动分析以及16SrDNA基因序列分析等方法多尺度地鉴定目的菌株。【结果】定向筛选得到了一株高活力,无硫脱毛效率高的菌株。鉴定结果表明,该菌株为地衣芽孢杆菌属,故命名为地衣芽孢杆菌(Bacillus licheniformis)X-47。【结论】应用多相定位选育技术筛选出的菌株地衣芽孢杆菌(Bacillus licheniformis)X-47,产角蛋白酶活力高,脱毛效率高,对胶原作用力弱的特点,具有开发无硫脱毛生物助剂的潜力。

Screening and identification of a new Bacillus strain producing keratinase

Abstract: Objective] New wild strains, which were endowed with an effective depilation as their abilities to produce keratinase, were isolated to develop the bio-deliplatory agent in the sulfur-free tanning. Methods] A polyphase screening method was used to obtain the high-yielding wild strains. In this method, the sewage sample was obtained from the specific circumstance where the raw hides were stored, and then enriched by adding the defatted wool power as an inducer in the medium. Furthermore, the strains were screened via assessing the ability of the depilation of their fermentation broth. Finally, the isolated strain was identified according to their morphological features, physiological and biochemical characteristics, automatic identification by Biolog system as well as phylogenetic analysis of 16SrDNA sequences. Results] A higher activity and remarkable dehairing capability with sulfur-free was obtained. The results of identification indicated that the strain X-47 was the most closely related to Bacillus licheniformis, so it was named Bacillus licheniformis X-47. Conclusion] The wild strain Bacillus licheniformis X-47 was isolated successfully by the method of polyphase screening. It had a high keratinase-producing activity, high efficient of depilation and weak effect on collagen, which suggested that it has a considerable potential of developing the sulfur-free bio-depilation product.