| 一株高效异养硝化菌的选育、鉴定及其硝化条件 |
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| 引用本文： | 曾庆梅,司文攻,李志强,靳靖,吴聪,魏春燕,黄博英.一株高效异养硝化菌的选育、鉴定及其硝化条件[J].微生物学报,2010,50(6):803-810. |
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| 作者姓名： | 曾庆梅 司文攻 李志强 靳靖 吴聪 魏春燕 黄博英 |
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| 作者单位： | 合肥工业大学,农产品生物化工教育部工程研究中心,合肥,230009 |
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| 基金项目： | 国家自然科学基金项目（30871739，30571304）；安徽省教育厅重点科研项目（KJ2007A099） |
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| 摘 要： | 【目的】针对现阶段异养硝化菌硝化速率较低的问题,选育更高效的异养硝化菌,进而鉴定该菌株的种属,了解其硝化特性和硝化条件。【方法】分别从污水处理厂活性污泥、化肥厂土壤以及农田土壤中取样,以柠檬酸钠为碳源,NH4Cl为氮源,采用污泥驯化、驯化过程中驯化液连续梯度稀释、平板划线分离及颜色指示剂快速硝化效果检测等步骤,筛得一株高效的异养硝化菌。经生理生化和16SrDNA序列的系统发育分析鉴定其种属;将该菌接入人工氨氮废水,定时检测水中含氮化合物的变化,了解其硝化特性;通过改变培养基碳源、溶氧量、C/N比、温度和pH考察其硝化条件。【结果】获得的高效异养硝化菌为革兰氏阴性杆菌,不利用葡萄糖发酵,氧化酶、接触酶阳性,不产吲哚,能由有机酸盐产碱;其与产碱菌属菌株Alcaligenessp.ES-SDK-3的16SrDNA同源性高达99.7%。用该菌株处理初始氨氮浓度为182.30mg/L的废水,30h后氨氮去除率为99.8%,指数期平均氨氮去除速率为9.61mg-N/L/h,其在硝化过程中几乎没有亚硝酸盐氮和硝酸盐氮产生;最佳碳源为柠檬酸钠;高的溶氧量和高的C/N比有利于其降解氨氮,当C/N比为12时即可达到较好的效果;该菌株在温度为30℃-35℃,pH为5.0-9.0范围内均能较彻底地降解氨氮。【结论】该菌株为产碱菌属,命名为Alcaligenessp.HN-S;其在硝化速率与处理的氨氮浓度方面均高于目前国内外筛出的大多数异养硝化菌;通过考察其硝化条件,为其走向实际污水脱氮工艺提供了依据。
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| 关 键 词： | 关键词：异养硝化菌 分离 鉴定 硝化作用 |
| 收稿时间： | 2009/11/9 0:00:00 |
| 修稿时间： | 2/6/2010 12:00:00 AM |
Isolation, identification and characterization of a high-efficient heterotrophic nitrifier |
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| Qingmei Zeng,Wengong Si,Zhiqiang Li,Jing Jin,Cong Wu,Chunyan Wei and Boying Huang.Isolation, identification and characterization of a high-efficient heterotrophic nitrifier[J].Acta Microbiologica Sinica,2010,50(6):803-810. |
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| Authors: | Qingmei Zeng Wengong Si Zhiqiang Li Jing Jin Cong Wu Chunyan Wei and Boying Huang |
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| Institution: | Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China;Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China;Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China;Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China;Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China;Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China;Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China |
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| Abstract: | |
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| Keywords: | Keywords: heterotrophic nitrifier isolation identification nitrification |
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