| 金黄色葡萄球菌稳定L型DNA的提取及酶切 |
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| 引用本文： | 何杰,张世馥,蒋玖,宋秀宇,何天源.金黄色葡萄球菌稳定L型DNA的提取及酶切[J].中国微生态学杂志,1994(4). |
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| 作者姓名： | 何杰 张世馥 蒋玖 宋秀宇 何天源 |
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| 作者单位： | 蚌埠医学院病理学教研室，蚌埠医学院微生物学教研室，蚌埠医学院生化学教研室 |
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| 摘 要： | 本文从一例恶性淋巴瘤合并金黄色葡萄球菌Ｌ型败血症患者的骨髓内分离得到金黄色葡萄球菌稳定Ｌ型，用玻璃微珠机械研磨方法提取ＤＮＡ，纯化后被ＥＣＯＲＩ酶切，酶切ＤＮＡ片段可用于核酸杂交等分子物生学研究。
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| 关 键 词： | 金黄色葡萄球菌L型，DNA，ECORI |
EXTRACTION ANG DIGESTION DNA FROM STAPHYLOCOCCUS AUREUS STABLE L-FORMS |
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| He Jei,et al..EXTRACTION ANG DIGESTION DNA FROM STAPHYLOCOCCUS AUREUS STABLE L-FORMS[J].Chinese Journal of Microecology,1994(4). |
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| Authors: | He Jei |
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| Institution: | Bengbu Medical College Anhui 233003 |
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| Abstract: | Straphylococcus aureus stable L-form DNA, which came from a pat-ient's marrow with malignent lymphoma accompanied straphylococcus au reus L-forms septicemia was extracted useing grinding method of glass pearl.After purification with ss-phenol/chloro form, the DNA could be digestedby EcoRI and the fragments,and applied to the hybridizati-on of DNA and other study in molecular biology. |
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| Keywords: | Staphylococcus aureus stable L-form DNA EcoRI |
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