| PCR-DGGE技术评价抗生素诱导的肠道菌群失调 |
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| 引用本文： | 况炜,唐欢,袁静,张志学,魏泓.PCR-DGGE技术评价抗生素诱导的肠道菌群失调[J].中国微生态学杂志,2008,20(5). |
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| 作者姓名： | 况炜 唐欢 袁静 张志学 魏泓 |
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| 作者单位： | 1. 西南大学生命科学学院,重庆,400715;第三军医大学,基础部实验动物学教研室,重庆,400038
2. 第三军医大学,基础部实验动物学教研室,重庆,400038 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家科技与支撑计划，国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的 利用PCR-DGGE技术结合活菌计数评价抗生素引起的肠道菌群失调.方法 SPF级BALB/c雌性小鼠连续4 d灌胃头孢曲松溶液125 mg/ml,0.4 ml/d,运用基于细菌16S DNA的PCR-DGGE技术结合活菌计数分析小鼠肠道菌群变化.结果 活菌计数结果与PCR-DGGE图谱显示,头孢曲松处理3 d后小鼠肠道菌群出现严重失调.结论 PCR-DGGE技术可直观而灵敏地显示抗生素处理过程中肠道菌群的动态变化,适用于评价抗生素引起的肠道菌群失调.
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| 关 键 词： | 肠道菌群失调 头孢曲松 BALB/c小鼠 |
Assessment of antibiotic-induced intestinal flora imbalance by PCR-DGGE |
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| KUANG Wei,TANG Huan,YUAN Jing,ZHANG Zhi-xue,WEI Hong.Assessment of antibiotic-induced intestinal flora imbalance by PCR-DGGE[J].Chinese Journal of Microecology,2008,20(5). |
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| Authors: | KUANG Wei TANG Huan YUAN Jing ZHANG Zhi-xue WEI Hong |
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| Abstract: | Objective To assess the ceftriaxone-induced intestinal flora imbalance by PCR-DGGE method and plate count.Methods Female SPF BALB/c mice were treated with ceftriaxone in a dose of 125 mg/ml,0.4 ml/d by oral gavage for four days.The amount of fecal dominant bacterium were quantified by viable count,and diversity of microflora was detected by 16S DNA-based PCR-DGGE.Results Both plate count and PCR-DGGE profile indicated that dysbacteria could be induced by administering ceftriaxone continually for 3 days.Conclusion PCR-DGGE can directly and sensitively display the dynamic succession of mice intestinal flora during the antibiotic treated process,which makes it an efficient method to assess the antibiotic-induced intestinal flora imbalance. |
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| Keywords: | PCR-DGGE |
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